CAJ | 학술논문

以花烛品种Amigo为材料,研究了悬浮培养条件下花烛体细胞胚胎发生过程中相关生理生化特征.结果表明:POD、CAT在胚性愈伤组织阶段维持较高活性,而SOD在体胚发育后期阶段活性较高;可溶性蛋白质含量在胚性愈伤组织阶段出现高峰;可溶性糖含量变化表现为先上升后下降的趋势,而淀粉含量表现为先下降后上升的趋势;SDS-PAGE电泳分析表明,胚性愈伤组织阶段蛋白质表达量高,种类多,并出现多种特异蛋白.分析认为胚性愈伤组织阶段是调控花烛体细胞胚胎发生过程的关键阶段.
이화충품충Amigo위재료,연구료현부배양조건하화충체세포배태발생과정중상관생리생화특정.결과표명:POD、CAT재배성유상조직계단유지교고활성,이SOD재체배발육후기계단활성교고;가용성단백질함량재배성유상조직계단출현고봉;가용성당함량변화표현위선상승후하강적추세,이정분함량표현위선하강후상승적추세;SDS-PAGE전영분석표명,배성유상조직계단단백질표체량고,충류다,병출현다충특이단백.분석인위배성유상조직계단시조공화충체세포배태발생과정적관건계단.
Using Anthurium andraeanum cv.'Amigo'as material,some physiological and biochemical characteristics of different developmental stages of somatic embryogenesis were studied. The results showed that the activity of POD and CAT maintained at a high level at the stage of embryogenic callus,and SOD activity present a high level at the later stage of somatic embryogenesis. The content of soluble protein was the highest at the stage of callus formation,and the content of starch went down first and up later but the content of soluble sugar up first and down later contrarily during the somatic embryogenesis. SDS-PAGE analysis showed that there are some specific proteins and high expression proteins at the stage of embryogenic callus. It concluded that the embryogenic callus formation stage is important for the regulation of somatic embryogenesis in A.andraeanum.