CAJ | 학술논문

背景:类风湿性关节炎的发生发展与滑膜细胞及淋巴细胞的增殖与凋亡不平衡密切相关,其滑膜细胞存在细胞凋亡过程的异常.目的:观察异种、异基因双份脐血干细胞移植对Ⅱ型胶原性关节炎小鼠Bcl-2 和Bax 表达的影响.方法:弗氏完全佐剂+Ⅱ型胶原诱导C57BL/6(H-2b)小鼠,建立Ⅱ型胶原性关节炎小鼠模型.小鼠二次免疫接种后第2 天,模型组、正常对照组尾静脉注射生理盐水,细胞移植组将脐血造血干细胞注入小鼠尾静脉内(其中单份剂量2×106/50 g;双份剂量:每份1×106/50 g,共2×106 /50 g).甲氨喋呤阳性对照组小鼠灌胃甲氨蝶呤,每次0.017 5 g/kg,每5 天1 次,共6 次.结果与结论:移植后第42 天全部处死动物取膝及肘以下关节组织,病理组织学显示正常对照组关节面光滑,滑膜层未见炎性细胞浸润,软骨细胞形态正常;模型组滑膜组织高度增生,大量的炎性细胞浸润,软骨面破坏;甲氨蝶呤阳性对照组、单份脐血造血干细胞移植组滑膜组织可见轻度增生,少量炎性细胞浸润,双份脐血造血干细胞移植组关节软骨表面光滑,未见破坏,有极少量炎性细胞浸润.免疫组织化学检测显示,双份脐血造血干细胞移植组Bax、Bcl-2 的表达低于单份脐血造血干细胞移植治疗组(P < 0.05);与正常对照组比较,差异无显著性意义(P >0.05).结果说明双份脐血干细胞在一定数量及作用时间内可诱导Ⅱ型胶原性关节炎小鼠滑膜细胞凋亡,对滑膜组织损伤起保护作用.
배경:류풍습성관절염적발생발전여활막세포급림파세포적증식여조망불평형밀절상관,기활막세포존재세포조망과정적이상.목적:관찰이충、이기인쌍빈제혈간세포이식대Ⅱ형효원성관절염소서Bcl-2 화Bax 표체적영향.방법:불씨완전좌제+Ⅱ형효원유도C57BL/6(H-2b)소서,건립Ⅱ형효원성관절염소서모형.소서이차면역접충후제2 천,모형조、정상대조조미정맥주사생리염수,세포이식조장제혈조혈간세포주입소서미정맥내(기중단빈제량2×106/50 g;쌍빈제량:매빈1×106/50 g,공2×106 /50 g).갑안첩령양성대조조소서관위갑안접령,매차0.017 5 g/kg,매5 천1 차,공6 차.결과여결론:이식후제42 천전부처사동물취슬급주이하관절조직,병리조직학현시정상대조조관절면광활,활막층미견염성세포침윤,연골세포형태정상;모형조활막조직고도증생,대량적염성세포침윤,연골면파배;갑안접령양성대조조、단빈제혈조혈간세포이식조활막조직가견경도증생,소량염성세포침윤,쌍빈제혈조혈간세포이식조관절연골표면광활,미견파배,유겁소량염성세포침윤.면역조직화학검측현시,쌍빈제혈조혈간세포이식조Bax、Bcl-2 적표체저우단빈제혈조혈간세포이식치료조(P < 0.05);여정상대조조비교,차이무현저성의의(P >0.05).결과설명쌍빈제혈간세포재일정수량급작용시간내가유도Ⅱ형효원성관절염소서활막세포조망,대활막조직손상기보호작용.
BACKGROUND: The occurrenceand development of rheumatoid arthritis were strongly associated with unbalance proliferation and apoptosis of synovial cells and lymphocytes. Some synovial cell apoptosis was abnormal. OBJECTIVE: To observe effects of heterogenic double umbilical cord blood stem cell transplantation on Bcl-2 and Bax expression in mice with type Ⅱcollagen-induced arthritis. METHODS: C57BL/6(H-2b) mice were induced with Freund's complete adjuvant and type Ⅱcollagen to establish mouse models of type Ⅱcollagen-induced arthritis.At 2days after secondary immunity incubation, mice were injected with saline via tail vein in the model and normal control groups. Umbilical cord blood hemopoietic stem cells were injected into mouse tail vein in the UBSCs transplantation groups (single dose:2×106/50 g; double dose: 1×106/50 g each, totally 2×106/50 g). Mice were intragastrically administrated methotrexate in the methotrexate positive control group, 0.017 5 g/kg once, once every 5 days, totally six times. RESULTS AND CONCLUSION: Joint tissue below knee and elbow was obtained at42 days following transplantation. Histopathology displayed that smooth and glossy articular surface, no inflammatory cell infiltration in the synovial layer and normal chondrocytes in the normal control group. Hyperplasia, a lot of inflammatory cell infiltration and damaged cartilage surface were visible in the model group. Slight hyperplasia and a little inflammatory cell infiltration were detectable in the methotrexate positive controlgroup and single UBSCs transplantation group. Double UBSCs transplantation group exhibited smooth and glossy articular cartilage surface, no damage, a little inflammatory cell infiltration. Immunohistochemistry demonstrated that Bcl-2 and Bax expression was lower in the double UBSCs transplantation group compared with single UBSCs transplantation group (P < 0.05). Compared with normal control group, no significant difference was detected (P > 0.05). Results suggest that double umbilical cord blood stem cells can induce synovial cell apoptosis in mice with type Ⅱcollagen-induced arthritis in a certain number and action time, and protect synovial membrane against damage.