CAJ | 학술논문

目的观察经胶原膜包裹或应用免疫抑制剂后,鸡羽根角蛋白(CCK)材料在体埋植对周围组织的影响.方法 30只SD大鼠,随机均分为5组.将经特殊处理的CCK埋植入竖脊肌组织中.实验组:A组为CCK材料埋植加术后两周腹腔内注射免疫抑制剂环孢素A(CsA,日剂量5 mg/kg),B组为CCK材料浸泡CsA(2.5 mg/ml)后埋植,C组为胶原膜包裹CCK材料后埋植,D组仅埋植CCK材料.空白对照组仅作切口,不埋植材料.分别于术后2、4、8周取材,常规切片染色,光镜下观察埋植材料的降解情况及对周围组织的影响,免疫组织化学术检测周围组织内T淋巴细胞浸润情况.结果术后各组大鼠一般状况良好,术后第2、4、8周,可见大量巨噬细胞,尤其是多核巨细胞分布于材料边缘,吞噬材料.炎性细胞数量较少.第2、4、8周A组CD3阳性表达细胞数较B组少,与D组有显著差异(P＜0.05),A组与空白对照组无显著差异(P＞0.05).结论短期应用免疫抑制剂可显著改善CCK的在体组织相容性;CCK在体内可降解,巨噬细胞,尤其多核巨细胞参与了该过程.
목적 관찰경효원막포과혹응용면역억제제후,계우근각단백(CCK)재료재체매식대주위조직적영향.방법 30지SD대서,수궤균분위5조.장경특수처리적CCK매식입수척기조직중.실험조:A조위CCK재료매식가술후량주복강내주사면역억제제배포소A(CsA,일제량5 mg/kg),B조위CCK재료침포CsA(2.5 mg/ml)후매식,C조위효원막포과CCK재료후매식,D조부매식CCK재료.공백대조조부작절구,불매식재료.분별우술후2、4、8주취재,상규절편염색,광경하관찰매식재료적강해정황급대주위조직적영향,면역조직화학술검측주위조직내T림파세포침윤정황.결과 술후각조대서일반상황량호,술후제2、4、8주,가견대량거서세포,우기시다핵거세포분포우재료변연,탄서재료.염성세포수량교소.제2、4、8주A조CD3양성표체세포수교B조소,여D조유현저차이(P＜0.05),A조여공백대조조무현저차이(P＞0.05).결론 단기응용면역억제제가현저개선CCK적재체조직상용성;CCK재체내가강해,거서세포,우기다핵거세포삼여료해과정.
Objective To improve the histocompatibility of chicken calamus keratin (CCK) graft by collagen-gel coating or using ofcyclosporine A (CsA). Methods Thirty SD rats were equally randomized into 5 groups, and in 4 of them, CCK implantation into the bilateral erector spinae was performed on different treatment protocols. In group A, the rats received daily intraperitoneal injection of CsA (5 mg/kg) for two consecutive weeks after CCK implantation; in group B, CCK was soaked in CsA (2.5 mg/ml) solution at 4℃ for 48 h before grafting; in group C, CCK coated with collagen gel was grafted;and in group D, only CCK was implanted. Rats in the fifth group received only cutaneous incision as well as muscular dissection to serve as the blank control. CCK degradation and its effect on the surrounding tissues were observed at 2, 4 and 8weeks after grafting. Immunohistochemistry was performed to identify T lymphocyte infiltration in the host tissues. Results All the rats survived the operation. Numerous macrophages, especially multinucleated giant cells occurred on the peripheral of the CCK grafts, and small degraded CCK pieces were observed in their cytoplasm. Only a few inflammatory cells were seen in the host tissues. At 2, 4 and 8 weeks after CCK implantation, only a few CD3-positive cells were found in all the groups, and in group A and B, the density of T lymphocytes was significantly lower than that in group D, and there was no significant difference between group A and the blank control group. Conclusions CsA significantly improves the histocompatibility of CCK material, and short-term systemic CsA administration achieves the best results. Macrophages, especially multinucleated giant cells participate in CCK degradation in vivo.