CAJ | 학술논문

目的为了解广东地区结核分枝杆菌rpoB基因突变特点,并探讨其与利福平耐药之间的关系.方法本研究根据结核病诊断细菌学检验规程从临床结核病疑似患者痰液中分离鉴定结核分枝杆菌,采用绝对浓度法对其进行药敏试验,并用PCR-DNA直接测序法对结核分枝杆菌rpoB基因进行序列分析.结果结果分离得到利福平耐药株27株,其中低浓度耐药8株,高浓度耐药19株.经测序分析,所有利福平敏感株rpoB基因均未发生氨基酸突变;利福平耐药株rpoB基因的突变率为81.5%(22/27),突变均发生在利福平耐药决定区内,突变位点为526、531及533,以Ser531Leu突变最为常见(55.6%,15/27),并且高浓度耐药株的突变率明显高于低浓度耐药株.结论结果表明广东省结核分枝杆菌rpoB基因突变是结核分枝杆菌利福平耐药性产生的主要分子机制,突变位点同国内外研究基本一致,但各位点突变形式所占比例有自身特点.
목적 위료해엄동지구결핵분지간균rpoB기인돌변특점,병탐토기여리복평내약지간적관계.방법 본연구근거결핵병진단세균학검험규정종림상결핵병의사환자담액중분리감정결핵분지간균,채용절대농도법대기진행약민시험,병용PCR-DNA직접측서법대결핵분지간균rpoB기인진행서렬분석.결과 결과분리득도리복평내약주27주,기중저농도내약8주,고농도내약19주.경측서분석,소유리복평민감주rpoB기인균미발생안기산돌변;리복평내약주rpoB기인적돌변솔위81.5%(22/27),돌변균발생재리복평내약결정구내,돌변위점위526、531급533,이Ser531Leu돌변최위상견(55.6%,15/27),병차고농도내약주적돌변솔명현고우저농도내약주.결론 결과표명광동성결핵분지간균rpoB기인돌변시결핵분지간균리복평내약성산생적주요분자궤제,돌변위점동국내외연구기본일치,단각위점돌변형식소점비례유자신특점.
Objective To study the characteristics of rpoB gene mutations of Mycobacterium tuberculosis in Guangdong province. Methods The drug susceptibility profiles were evaluated by absolute concentration method and genetic mutations on rpoB gene were identified by DNA direct sequencing. Results In our study, 27 rifampin resistant were found, M. tuberculosis were obtained, including 8 low level resistant isolates and 19 high level resistant isolates, All of the rifampin susceptible isolates were found to harbor no detectable mutations. 81.5% of rifampinresistant strains were found to have mutations in the analyzed pro B,which located at codon 526, 531 and533, and the most frequent mutation pattern was Ser531Leu (55.6%).The frequency of rpoB mutations of high level resistant isolates was higher than those of low level resistant isolates. Conclusion In conclusions, mutations of rpoB gene were the main molecular mechanism of rifampin resistant M. tuberculosis. The mutation sites of rifampin resistant strains correspond with current studies, but the proportion of each mutation form has their own characteristics in our study.