CAJ | 학술논문

目的进行一株新疆维吾尔族D型HBV的全基因克隆.方法应用PCR扩增HBV全基因,进行全基因克隆,挑取阳性克隆进行序列测定,使用BioEdit和美国国立生物技术信息中心(NCBI)-BLAST工具进行生物信息学分析.结果获得1例新疆维吾尔族慢性HBV感染者D型HBV病毒株的全基因序列,其基因组全长为3174 bp,与目前D基因型参照序列比较,同源性为92%～98%,与1株来自中国的DC重组基因型(AY862865)比较,同源性为91%.进一步分析发现,该新疆病毒株与目前D基因型基因库参照序列比较,有9个碱基缺失,位于核苷酸第1760～1768位(ATTAAAGGT),即可能发生了缺失突变.氨基酸分析发现,其血清型为ayw2型.重组位点分析发现,该新疆病毒株未与其他基因型重组.该新疆株与2株来自土耳其的HBV株(AY796032和AY721605)遗传距离最近.结论成功克隆了一例维吾尔族慢性HBV感染者D型HBV病毒株的全基因序列,新疆维吾尔族D基因型HBV病毒株有其自身特点.
목적 진행일주신강유오이족D형HBV적전기인극륭.방법응용PCR확증HBV전기인,진행전기인극륭,도취양성극륭진행서렬측정,사용BioEdit화미국국립생물기술신식중심(NCBI)-BLAST공구진행생물신식학분석.결과획득1례신강유오이족만성HBV감염자D형HBV병독주적전기인서렬,기기인조전장위3174 bp,여목전D기인형삼조서렬비교,동원성위92%～98%,여1주래자중국적DC중조기인형(AY862865)비교,동원성위91%.진일보분석발현,해신강병독주여목전D기인형기인고삼조서렬비교,유9개감기결실,위우핵감산제1760～1768위(ATTAAAGGT),즉가능발생료결실돌변.안기산분석발현,기혈청형위ayw2형.중조위점분석발현,해신강병독주미여기타기인형중조.해신강주여2주래자토이기적HBV주(AY796032화AY721605)유전거리최근.결론성공극륭료일례유오이족만성HBV감염자D형HBV병독주적전기인서렬,신강유오이족D기인형HBV병독주유기자신특점.
Objective To determine the complete nucleotide sequence of a genotype D hepatitis B virus (HBV) strain from a Uighur patient with chronic hepatitis B in Xinjiang.Methods The complete nucleotide sequence of a HBV strain obtained from a Uighur patient with chronic hepatitis B was amplified by polymerase chain reaction (PCR) and cloned for sequencing.The bioinformatics analysis was done using BioEdit and National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI-BLAST).Results The complete nucleotide sequence of Xinjiang HBV strain was 3174 bp.The nucleotide homology was 92%-98% between the Xinjiang strain and the published genotype D HBV strains and 91% compared to the published genotype D/C combination HBV strains from China (AY862865).There was a 9 bp deletion from nucleotide 1760 to nucleotide 1768 (ATTAAAGGT).This Xinjiang HBV strain belonged to ayw2 subtype and D genotype without any recombination with other genotypes.In the term of evolutionary relation,the complete nucleotide sequence of this Xinjiang HBV strain was closest to those of two Turkey HBV strains (AY796032 and AY721605) among 34 genotype D strains published in GenBank.Conclusions The complete nucleotide sequence of a genotype D HBV strain from a Uighur chronic hepatitis B patient in Xinjiang is determined.The sequence shows some unique characteristics.