中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2010年
10期
949-952
,共4页
姚庆和%张华%高国栋%张鹏
姚慶和%張華%高國棟%張鵬
요경화%장화%고국동%장붕
癫痫%电刺激%大脑皮质%模型,动物%大鼠
癲癇%電刺激%大腦皮質%模型,動物%大鼠
전간%전자격%대뇌피질%모형,동물%대서
Epilepsy%Electric stimulation%Cerebral coretex%Models,animal%Rat
目的 观察重复脑皮质电刺激对氯化铁诱发慢性癫痫大鼠模型脑皮质兴奋性的影响.方法 通过在运动感觉区脑皮质注射氯化铁建立慢性癫痫大鼠模型,给予脑皮质低频(1 Hz)低强度(0.1 mA)和低频(1 Hz)高强度(1.0 mA)、高频(100 Hz)低强度(0.1 mA)和高频(100 Hz)高强度(1.0 mA)不同的重复电刺激,检测电刺激前后脑皮质后放电阈值、后放电时程和行为学评分.假刺激慢性癫痫大鼠作为对照组.结果 后放电阈值低频低强度组(2.10±0.38)mA与对照组(1.50±0.33)mA相比差异有统计学意义(P＜0.05).行为学评分和后放电时程各组与对照组相比差异无统计学意义.行为学评分与后放电阈值的比值低频低强度组(1.88±0.60)和低频高强度组(2.18±0.38)与对照组(3.22±0.67)相比差异有统计学意义(P＜0.01和P＜0.05).结论 重复低频低强度脑皮质电刺激可以升高氯化铁诱发慢性癫痫大鼠模型的脑皮质后放电阈值,降低脑皮质兴奋性,提示合适参数的脑皮质电刺激对氯化铁诱发大鼠癜痫具有抑制作用.
目的 觀察重複腦皮質電刺激對氯化鐵誘髮慢性癲癇大鼠模型腦皮質興奮性的影響.方法 通過在運動感覺區腦皮質註射氯化鐵建立慢性癲癇大鼠模型,給予腦皮質低頻(1 Hz)低彊度(0.1 mA)和低頻(1 Hz)高彊度(1.0 mA)、高頻(100 Hz)低彊度(0.1 mA)和高頻(100 Hz)高彊度(1.0 mA)不同的重複電刺激,檢測電刺激前後腦皮質後放電閾值、後放電時程和行為學評分.假刺激慢性癲癇大鼠作為對照組.結果 後放電閾值低頻低彊度組(2.10±0.38)mA與對照組(1.50±0.33)mA相比差異有統計學意義(P＜0.05).行為學評分和後放電時程各組與對照組相比差異無統計學意義.行為學評分與後放電閾值的比值低頻低彊度組(1.88±0.60)和低頻高彊度組(2.18±0.38)與對照組(3.22±0.67)相比差異有統計學意義(P＜0.01和P＜0.05).結論 重複低頻低彊度腦皮質電刺激可以升高氯化鐵誘髮慢性癲癇大鼠模型的腦皮質後放電閾值,降低腦皮質興奮性,提示閤適參數的腦皮質電刺激對氯化鐵誘髮大鼠癜癇具有抑製作用.
목적 관찰중복뇌피질전자격대록화철유발만성전간대서모형뇌피질흥강성적영향.방법 통과재운동감각구뇌피질주사록화철건립만성전간대서모형,급여뇌피질저빈(1 Hz)저강도(0.1 mA)화저빈(1 Hz)고강도(1.0 mA)、고빈(100 Hz)저강도(0.1 mA)화고빈(100 Hz)고강도(1.0 mA)불동적중복전자격,검측전자격전후뇌피질후방전역치、후방전시정화행위학평분.가자격만성전간대서작위대조조.결과 후방전역치저빈저강도조(2.10±0.38)mA여대조조(1.50±0.33)mA상비차이유통계학의의(P＜0.05).행위학평분화후방전시정각조여대조조상비차이무통계학의의.행위학평분여후방전역치적비치저빈저강도조(1.88±0.60)화저빈고강도조(2.18±0.38)여대조조(3.22±0.67)상비차이유통계학의의(P＜0.01화P＜0.05).결론 중복저빈저강도뇌피질전자격가이승고록화철유발만성전간대서모형적뇌피질후방전역치,강저뇌피질흥강성,제시합괄삼수적뇌피질전자격대록화철유발대서전간구유억제작용.
Objective To study the effects of repetitive electric cortical stimulation on the cortical excitability in the chronic epileptic rats induced by ferric chloride. Methods The chronic epileptic rats induced by intracortical injection of ferric chloride solution in the sensorimotor area were used. So we studied the effects of the low frequency (1 Hz) low amplitude (0.1 mA)and low frequency (1 Hz)high amplitude (1.0 mA)and high frequency(100 Hz)low amplitude(0.1 mA)and high frequency(100 Hz)high amplitude(1.0 mA)electric cortical stimulation on the cortical excitability by investigated the threshold and duration of cortical after discharge and the behavior score in the chronic epileptic rat. At the same time rats were treated with the sham-stimulation as control group. Results After 1 Hz and 0.1 mA repetitive electric cortical stimulation in 5 d, the cortical afterdischarge threshold (2.10 ±0.38)mA significantly ascended compared with that of the control group (1.5 ±0.33) mA, P ＜ 0.05. There was no statistic difference of behavior score and duration of cortical afterdischarge after repetitive electric cortical stimulation in 5 d compared with that of the control group. The ratio of behavior score and cortical afterdischarge threshold after 1 Hz and 0. 1 mA (1.88 ±0.60) or 1 Hz and 1.0 mA (2.18 ±0.38) repetitive electric cortical stimulation in 5 d was significantly lower than that of the control group (3.22 ±0.67, P ＜0.01 and P ＜0.05, respectively). Conclusion After the repetitive low frequency(1 Hz)low amplitude (0.1 mA)electric cortical stimulation the cortical afterdischarge threshold significantly ascended in the chronic epileptic rat induced by intracortical injection of ferric chloride solution. It means the depression of cortical excitability. The results of this study showed that electric cortical stimulation with suitable parameter can produce a suppressive effect on the epileptic rat induced by intracortical injection of ferric chloride solution.
