中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
3期
34-36
,共3页
宫颈癌%宫颈上皮内瘤变%Bcl-2基因%人乳头瘤病毒
宮頸癌%宮頸上皮內瘤變%Bcl-2基因%人乳頭瘤病毒
궁경암%궁경상피내류변%Bcl-2기인%인유두류병독
Cervical carcinoma%CIN%Bcl-2gene%HPV
目的 研究宫颈癌组织Bcl-2基因的表达与人乳头瘤病毒(HPV)感染的关系.方法 应用免疫组化PV-9000法对正常宫颈组织38例、各级宫颈上皮内瘤样变(CIN)组织106例、宫颈癌组织66例中Bcl-2基因的表达水平进行检测.应用PCR方法对66例宫颈癌组织中HPV表达进行检测及分型.结果 Bcl-2在各宫颈组织中均有表达,宫颈癌组Bcl-2阳性表达率(57.6%),显著高于正常宫颈组(15.8%)和CINⅠ组(19.4%),P均<0.01;高于CINⅡ组(28.6%),P＜0.05;与CINⅢ组(60.0%)比较差异无统计学意义,P＞0.05.宫颈癌组织中Bcl-2蛋白表达与HPV感染具有相关性,P＜0.05.Bcl-2蛋白在HPV16/18阳性宫颈癌组织和HPV其他型别阳性宫颈癌组织中表达差异无统计学意义,P＞0.05.结论 Bcl-2蛋白的高表达可能在宫颈癌的发生发展中起重要作用.Bcl-2蛋白的高表达与宫颈癌HPV感染相关,但与HPV型别关系不大.Bcl-2蛋白可能成为宫颈癌诊断和治疗的指标.
目的 研究宮頸癌組織Bcl-2基因的錶達與人乳頭瘤病毒(HPV)感染的關繫.方法 應用免疫組化PV-9000法對正常宮頸組織38例、各級宮頸上皮內瘤樣變(CIN)組織106例、宮頸癌組織66例中Bcl-2基因的錶達水平進行檢測.應用PCR方法對66例宮頸癌組織中HPV錶達進行檢測及分型.結果 Bcl-2在各宮頸組織中均有錶達,宮頸癌組Bcl-2暘性錶達率(57.6%),顯著高于正常宮頸組(15.8%)和CINⅠ組(19.4%),P均<0.01;高于CINⅡ組(28.6%),P＜0.05;與CINⅢ組(60.0%)比較差異無統計學意義,P＞0.05.宮頸癌組織中Bcl-2蛋白錶達與HPV感染具有相關性,P＜0.05.Bcl-2蛋白在HPV16/18暘性宮頸癌組織和HPV其他型彆暘性宮頸癌組織中錶達差異無統計學意義,P＞0.05.結論 Bcl-2蛋白的高錶達可能在宮頸癌的髮生髮展中起重要作用.Bcl-2蛋白的高錶達與宮頸癌HPV感染相關,但與HPV型彆關繫不大.Bcl-2蛋白可能成為宮頸癌診斷和治療的指標.
목적 연구궁경암조직Bcl-2기인적표체여인유두류병독(HPV)감염적관계.방법 응용면역조화PV-9000법대정상궁경조직38례、각급궁경상피내류양변(CIN)조직106례、궁경암조직66례중Bcl-2기인적표체수평진행검측.응용PCR방법대66례궁경암조직중HPV표체진행검측급분형.결과 Bcl-2재각궁경조직중균유표체,궁경암조Bcl-2양성표체솔(57.6%),현저고우정상궁경조(15.8%)화CINⅠ조(19.4%),P균<0.01;고우CINⅡ조(28.6%),P＜0.05;여CINⅢ조(60.0%)비교차이무통계학의의,P＞0.05.궁경암조직중Bcl-2단백표체여HPV감염구유상관성,P＜0.05.Bcl-2단백재HPV16/18양성궁경암조직화HPV기타형별양성궁경암조직중표체차이무통계학의의,P＞0.05.결론 Bcl-2단백적고표체가능재궁경암적발생발전중기중요작용.Bcl-2단백적고표체여궁경암HPV감염상관,단여HPV형별관계불대.Bcl-2단백가능성위궁경암진단화치료적지표.
Objective To study the relationship between expression of Bcl-2 and HPV infection in cervical cancer. Methods The expression of Bcl-2 was detected by immunohistochemistry PV-9000 in 38 cases of benign squamous epithelia, 106 cases of cervical intraepithelial neoplasia, 66 cases of cervical carcinoma. The expression of HPV and it's tape was detected by PCR in 66 cases of cercival cancer. Results Bcl-2 were expressed in all of examples. The positive rate of Bcl-2 in cervical carcinoma (57.6%) was statistically different from benign squamous epithelia(15.8%)and CINⅠ(19.4%), P<0.01. The positive rate was higher in cervical carcinoma than in CINⅡ(28.6%),P＜0.05. The positive rate were similar in cervical carcinoma and CINⅢ(60.0%),P＞0.05.The relationship between the expression of Bcl-2 and HPV infection were significiant, P＜0.05. The expression of Bcl-2 was not different between HPV16/18 and other type of HPV in cervical cancer, P＞0.05. Conclusion The expression of Bcl-2 may play an important role in cervical arcinoma occurrence and development. The over-expression of Bcl-2 have signigificiant relationship with HPV infection. But it's expression are not correlated with the type of HPV. Bcl-2 may be a diagnosis and therapy, index of cervical cancer.
