南开大学学报(自然科学版)
南開大學學報(自然科學版)
남개대학학보(자연과학판)
ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NANKAIENSIS
2002年
4期
1-5
,共5页
祝建洪%孙建%陈珈%李桂兰%吴卓伟%王隆飞%陈功%Marc G Fortin%李明刚
祝建洪%孫建%陳珈%李桂蘭%吳卓偉%王隆飛%陳功%Marc G Fortin%李明剛
축건홍%손건%진가%리계란%오탁위%왕륭비%진공%Marc G Fortin%리명강
植酸酶%酿酒酵母%分泌
植痠酶%釀酒酵母%分泌
식산매%양주효모%분비
phytase%S. cerevisiae%secretion
通过逆转录PCR从无花果曲霉总RNA中扩增出1.4-kb带信号肽的植酸酶基因,将其克隆到穿梭表达载体pYES2,构建了含有目的基因的重组质粒pYPA1.用pYPA1转化酿酒酵母INVSc1菌株,成功地构建了能分泌活性植酸酶的工程菌株.该菌株的成功构建为饲料和食品添加剂以及解磷工程菌肥的开发奠定了基础.
通過逆轉錄PCR從無花果麯黴總RNA中擴增齣1.4-kb帶信號肽的植痠酶基因,將其剋隆到穿梭錶達載體pYES2,構建瞭含有目的基因的重組質粒pYPA1.用pYPA1轉化釀酒酵母INVSc1菌株,成功地構建瞭能分泌活性植痠酶的工程菌株.該菌株的成功構建為飼料和食品添加劑以及解燐工程菌肥的開髮奠定瞭基礎.
통과역전록PCR종무화과곡매총RNA중확증출1.4-kb대신호태적식산매기인,장기극륭도천사표체재체pYES2,구건료함유목적기인적중조질립pYPA1.용pYPA1전화양주효모INVSc1균주,성공지구건료능분비활성식산매적공정균주.해균주적성공구건위사료화식품첨가제이급해린공정균비적개발전정료기출.
A 1.4-kb phytase gene amplified by RT-PCR from A. ficuum total RNA was successfully cloned into theexpression vector pYES2, subsequently the recombinant expression vector pYPA1 was obtained. By thetransformation with pYPA1, a transgenic S. cerevisiae that could express extracellular functional phytase wasconstructed. The successful construction of this engineering yeast strain made the groundwork for research onfeed additive and microbial fertilizer.
