CAJ | 학술논문

本研究工作以福建九龙江口龙海红树林自然保护区浮宫种苗园内7种红树植物为材料，采用改进的CTAB法获得了纯度较高(A260/A280在1.6～2.0之间)，得率高(DNA平均得率约为120×10－6m/m，Fresh Weight)，片段完整(片段大小约为23kb左右)的基因组DNA，并运用RAPD技术对这7种红树植物进行了遗传多样性分析．从30个10-mer随机引物中筛选出9个有效引物，并利用这9个有效引物共扩增出352条DNA带.利用UPGMA法对红树植物的7个种间的亲缘关系进行聚类分析，得出7个种的DNA分子分类系统图,并与传统的分类进行比较,发现结果相符，从而确定了RAPD技术用于红树植物分子分类研究的可行性，并为从分子水平研究红树植物遗传多样性,保护、开发和利用红树林资源提供科学依据.
본연구공작이복건구룡강구룡해홍수림자연보호구부궁충묘완내7충홍수식물위재료，채용개진적CTAB법획득료순도교고(A260/A280재1.6～2.0지간)，득솔고(DNA평균득솔약위120×10－6m/m，Fresh Weight)，편단완정(편단대소약위23kb좌우)적기인조DNA，병운용RAPD기술대저7충홍수식물진행료유전다양성분석．종30개10-mer수궤인물중사선출9개유효인물，병이용저9개유효인물공확증출352조DNA대.이용UPGMA법대홍수식물적7개충간적친연관계진행취류분석，득출7개충적DNA분자분류계통도,병여전통적분류진행비교,발현결과상부，종이학정료RAPD기술용우홍수식물분자분류연구적가행성，병위종분자수평연구홍수식물유전다양성,보호、개발화이용홍수림자원제공과학의거.
DNAs from 7 species of mangroves were extracted by the CTAB method. The A260/A280 value of DNA solutions ranged from 1.6 to 2.0. RAPD analysises on genetic diversity of mangroves were conducted. 9 effective primers were screened from 30 arbitrary primers, and 352 DNA bands were amplified. Based on UPGMA cluster analysis on DNA bands amplified by 9 primers, a DNA molecular dendrogram was established. Compared to the conventional classification, the molecular classification of mangroves was accurate.