CAJ | 학술논문

对急性脑梗塞多种中医体质进行HLA-DQA1等位基因分型，分析体质类型的遗传易感性及体质与“证”和治疗的密切关系。采用PCR-SSP技术对观察组急性脑梗塞阴虚质、阳虚质、气虚质、痰湿质、血瘀质共103例及正常质对照组99例进行HLA-DQA1等位基因的基因分型。结果表明，HLA-DQA10501基因型在阴虚质明显高于正常质对照组(P＜0.01)，HLA-DQA10301基因型在气虚质、痰湿质、血瘀质均明显高于正常质对照组(P＜0.01)。提示急性脑梗塞患者，HLA-DQA10501基因与阴虚质相关联；HLA-DQA10301基因与气虚质、痰湿质及血瘀质相关联；从分子生物学水平阐明了体质与“证”，体质与“同病异治”的遗传学背景。
대급성뇌경새다충중의체질진행HLA-DQA1등위기인분형，분석체질류형적유전역감성급체질여“증”화치료적밀절관계。채용PCR-SSP기술대관찰조급성뇌경새음허질、양허질、기허질、담습질、혈어질공103례급정상질대조조99례진행HLA-DQA1등위기인적기인분형。결과표명，HLA-DQA10501기인형재음허질명현고우정상질대조조(P＜0.01)，HLA-DQA10301기인형재기허질、담습질、혈어질균명현고우정상질대조조(P＜0.01)。제시급성뇌경새환자，HLA-DQA10501기인여음허질상관련；HLA-DQA10301기인여기허질、담습질급혈어질상관련；종분자생물학수평천명료체질여“증”，체질여“동병이치”적유전학배경。
Varied TCM constitutions in the patient of acute cerebralinfarction were classifyed by using HLA-DQA1 allelic gene to analyze hereditary susceptibility of constitution types and relation among constitution, syndrome and treatment. PCR-SSP technique was used for classification of Yin-deficiency , Yang-deficiency, Qi-deficiency, phlegm-dampness and blood stasis constitution in 103 cases of acute cerebral infarction and HLA-DQA1 allelic gene was used for gene classification in 99 cases of nomal constituion. Results indicated that HLA-DQA*0501 gene type in Yin-deficiency constitution was significant higher than that of normal constitution(P<0.01); HLA-DQA1*0301 gene type in Qi-deficiency, phlegm-dampness and blood stasis constitution all was higher than normal constitution(P<0.01).It is suggested that HLA-DQA1*0501 gene is correlated with Yin-deficiency in the patient of acute cerebral infarction. Thus, genetical background of relation of constitution with syndrome,and constitution with“ treating the same disease with different methods”are explained from view of point of molecular biology.