CAJ | 학술논문

目的：建立检测丙型肝炎病毒相关多肽抗体(GOR抗体)的酶联免疫吸附试验(ELISA)。方法：以丙肝病毒GOR二段肽为包被抗原，建立检测抗-GOR的ELISA法。结果：批内、批间变异系数分别为5.9%、9.1%，阳性血清用兔抗-GOR作阻断试验，其抑制率与兔抗-GOR呈剂量依赖关系。与抗HBV-IgG、抗HCV-IgG、抗CMV-IgG、RF阳性血清无交叉反应性。经对60例慢性丙型肝炎、48例抗HCV-IgG阳性的无症状HCV感染者、63例慢性乙型肝炎、41例肝硬化、32例肝癌、12例白血病、39例血透患者检测，其GOR抗体阳性率分别为60.0%、52.1%、14.3%、31.7%、28.1%、50.0%、43.6%。结论：此法敏感、简便、快速，抗-GOR与丙型肝炎密切相关。
목적：건립검측병형간염병독상관다태항체(GOR항체)적매련면역흡부시험(ELISA)。방법：이병간병독GOR이단태위포피항원，건립검측항-GOR적ELISA법。결과：비내、비간변이계수분별위5.9%、9.1%，양성혈청용토항-GOR작조단시험，기억제솔여토항-GOR정제량의뢰관계。여항HBV-IgG、항HCV-IgG、항CMV-IgG、RF양성혈청무교차반응성。경대60례만성병형간염、48례항HCV-IgG양성적무증상HCV감염자、63례만성을형간염、41례간경화、32례간암、12례백혈병、39례혈투환자검측，기GOR항체양성솔분별위60.0%、52.1%、14.3%、31.7%、28.1%、50.0%、43.6%。결론：차법민감、간편、쾌속，항-GOR여병형간염밀절상관。
Objective:To establish an enzyme-linked immunosorbentassay(ELISA) for detecting anti-GOR antibody in various hepatic diseases.Methods:The sera were added to the polystyrene micro-titer plates coated with HCV relative synthetic peptide followed by adding HRP-conjugated goat anti-human IgG.Results:The within and between assay CV were 5.9% and 9.1% respectively.No cross-reaction was detected with anti-GOR for anti-HBc\,anti-HCV\,anti-CMV\,ANA,nor was there any interference by rheumatoid-factor.The anti-GOR positive rate in the patients with various diseases as well as the normal controls were measured.Conclusions:The assay was easy\,rapid and specific.It was found positive rate of anti-GOR was significanthy elevated in the various patients,and anti-GOR was correlative with infection of HCV.