浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2001年
2期
78-80
,共3页
王汉荣%茹水江%陈笑芸%贝亚维%顾秀慧%高春先
王漢榮%茹水江%陳笑蕓%貝亞維%顧秀慧%高春先
왕한영%여수강%진소예%패아유%고수혜%고춘선
无土栽培%根际%疫霉菌%离体检测
無土栽培%根際%疫黴菌%離體檢測
무토재배%근제%역매균%리체검측
经3年研究建立了一套用离体植物器官检测无土栽培系统中果菜类根际疫霉菌的简易方法,应用该方法检测120份营养液。其结果与实际符合率达到96.7%以上。经研究确定了该方法较合适的供测离体植物器官、取样量及较适的检测温度、光照等环境条件,并应用疫霉菌的静止子测定了该方法的灵敏度,最低可检测浓度为1×103个/ml;与其它方法相比,离体植物器官检测技术具有直观、简便、实用等优点,适合于广大基层技术人员和农民使用。
經3年研究建立瞭一套用離體植物器官檢測無土栽培繫統中果菜類根際疫黴菌的簡易方法,應用該方法檢測120份營養液。其結果與實際符閤率達到96.7%以上。經研究確定瞭該方法較閤適的供測離體植物器官、取樣量及較適的檢測溫度、光照等環境條件,併應用疫黴菌的靜止子測定瞭該方法的靈敏度,最低可檢測濃度為1×103箇/ml;與其它方法相比,離體植物器官檢測技術具有直觀、簡便、實用等優點,適閤于廣大基層技術人員和農民使用。
경3년연구건립료일투용리체식물기관검측무토재배계통중과채류근제역매균적간역방법,응용해방법검측120빈영양액。기결과여실제부합솔체도96.7%이상。경연구학정료해방법교합괄적공측리체식물기관、취양량급교괄적검측온도、광조등배경조건,병응용역매균적정지자측정료해방법적령민도,최저가검측농도위1×103개/ml;여기타방법상비,리체식물기관검측기술구유직관、간편、실용등우점,괄합우엄대기층기술인원화농민사용。
A series of inspection technique by plant organ in vitro test for phytophthora were established in a 3-year study.With the mehtod 120 samples of nutrient solution were successful inoculated onto crisp eggplant in vitro and produced lesious and hyphase and zoosporangia.Suitable plant organ for in vitro test,sample amount,temperature and other conditions were determined.The lowest concen tration of cystospore is 1×103 cells/ml,Compared with other mehtods,this method was more directly perceived on screen,more handy and simple,more stable and practical et al.