CAJ | 학술논문

研究了经0.23T～0.61T磁场处理1h所得磁化过氧化氢酶(CAT)的滞后活力、紫外差光谱及荧光发射光谱的变化。磁化酶在放置过程中，活力下降慢于天然酶，相对活力随滞后时间的延长而增加，活力变化曲线的线型随磁感应强度增加而呈现规律性变化。在滞后期间内，磁化酶紫外差光谱连续变化，荧光发射光谱λ 330 nm发射峰位未移动，而荧光发射强度下降。研究表明，磁化酶稳定性增加，其变性失活的构象变化过程明显不同于天然酶。同时，初步分析了磁化酶稳定性增加的机理。
연구료경0.23T～0.61T자장처리1h소득자화과양화경매(CAT)적체후활력、자외차광보급형광발사광보적변화。자화매재방치과정중，활력하강만우천연매，상대활력수체후시간적연장이증가，활력변화곡선적선형수자감응강도증가이정현규률성변화。재체후기간내，자화매자외차광보련속변화，형광발사광보λ 330 nm발사봉위미이동，이형광발사강도하강。연구표명，자화매은정성증가，기변성실활적구상변화과정명현불동우천연매。동시，초보분석료자화매은정성증가적궤리。
The hyteretic relative activity, ultraviolet differential spectra and fluorescence emission spectra of magnetized catalase exposed to 0.23～0.61 T magne tic field for 1 hour were studied. When allowed to stand in a non-magnetic envir onment, the magnetized enzyme activity dropped more slowly than that of the natu ral enzyme, the relative activity of the magnetized enzyme tended to grow and th e types of enzymatic activity curves showed regular changes. During the hysteret ic period the ultraviolet differential spectra changed continuously, the locatio n of fluorescence emission peak was not shifted, but its relative fluorescence i ntensity decreased. Such results indicated that the stability of the magnetized enzyme rose in contrast with the natural enzyme, and the process of its inactiva tion caused by conformational changes were distinctly different from those of th e natural enzyme. The possible mechanism for the increase in the stability of th e magnetic enzyme is discussed in the present paper.