CAJ | 학술논문

将鸭瘟粗提病毒经Sephadex G200柱层析纯化后作为包被抗原，以健康鸭IgG提纯后免疫羊，制备羊抗鸭IgG，并用过碘酸钠法进行辣根过氧化物酶标记，建立了检测鸭瘟病毒抗体的间接酶联免疫吸附试验(ELISA)。经对不同血清样品的检测，表明此方法特异性高，重复性好，操作简便，适于大批鸭群抗体水平监测和流行病学调查，为合理免疫和科学预防提供了技术手段。
장압온조제병독경Sephadex G200주층석순화후작위포피항원，이건강압IgG제순후면역양，제비양항압IgG，병용과전산납법진행랄근과양화물매표기，건립료검측압온병독항체적간접매련면역흡부시험(ELISA)。경대불동혈청양품적검측，표명차방법특이성고，중복성호，조작간편，괄우대비압군항체수평감측화류행병학조사，위합리면역화과학예방제공료기술수단。
An indirect enzyme-linked immunosorbent assay(ELISA)was established as following method.The crude extraction of plague virus was purified as coating antigen by means of sephadex-G200 chromatography.The goat was immunized by negative antisera from the ducks free from DPV for goat IgG against duck,which was labelled with horseradish peroxidase(HRP)by Nalo4.Maternal antibodies and adaptive immune antibodies from duckling and the sera sample from the ducks in different clinical immune Qtatus were examined by ELISA.The results showed that the method is high specificity and sensitivity for detection of antibodies against DPV，and can be used in clinical serological surveillance.