CAJ | 학술논문

目的探寻G蛋白信号传导途径中与Gβ亚基相互作用的下游蛋白因子，揭示G蛋白信号传导通路的机制。方法采用敏感性较高的酵母双杂交体系，构建含Gβ亚基基因的饵质粒筛选人脑cDNA文库。结果获得了肌动蛋白集束调控蛋白(actin bundling protein)的编码基因和两个新基因片段，GeneBank登录号分别为AF288405(427 bp)及AF288406(2832 bp)。结论提示在人脑组织中Gβ亚基作为结构和功能单位可能通过与actin bundling protein 及另两种未知的蛋白因子间的相互作用而介导了信号的传导，对于揭示G蛋白信号传递通路与actin细胞骨架之间的关系起重要的提示作用
목적탐심G단백신호전도도경중여Gβ아기상호작용적하유단백인자，게시G단백신호전도통로적궤제。방법채용민감성교고적효모쌍잡교체계，구건함Gβ아기기인적이질립사선인뇌cDNA문고。결과획득료기동단백집속조공단백(actin bundling protein)적편마기인화량개신기인편단，GeneBank등록호분별위AF288405(427 bp)급AF288406(2832 bp)。결론제시재인뇌조직중Gβ아기작위결구화공능단위가능통과여actin bundling protein 급령량충미지적단백인자간적상호작용이개도료신호적전도，대우게시G단백신호전체통로여actin세포골가지간적관계기중요적제시작용
Objective To explore the protein factors that could interact with Gβ subunit within the G protein sig nal transducing pathway. Methods The highly sensitive protein-protein interaction system——Yeast Two-hybrid System was applied to screen the human cDNA library with constructed “Bite plasmid” contain ing Gβ subunit gene fragment. And then the faulse positive test was adapted. Results Three positive gene fragments were obtained. One codes for “Actin bundling protein”. The other two are new ones and their GeneBank accession numbers are AF288405 and AF288406 respectively. Conclusions It is the first time to find that among human brain tissue, Gβ subunit becomes a structural or functional unit interacts with actin bundling protein and the other two unknown protein factors to activate the following pathway. This re sult may be important to understand the relationship between the actin cytoskeleton and G proteins.