CAJ | 학술논문

目的：探讨胃癌组织中细胞周期蛋白E的表达及其与胃癌临床病理因素的关系。方法：用免疫组织化学方法检测了40例胃癌组织中细胞周期蛋白E和p53的表达，用扩增角蛋白19mRNA的巢式RT-PCR方法检测周围静脉血微转移。结果：40例胃癌组织中细胞周期蛋白E表达阳性率为45.0%，其表达与肿瘤浸润深度、TNM分期及外周血微转移密切相关(P<0.05)。p53蛋白表达阳性率为52.5%，在细胞周期蛋白E阳性胃癌中p53阳性率明显高于细胞周期蛋白E阴性胃癌(P<0.05)。结论：细胞周期蛋白E在胃癌中的表达与p53的表达呈明显的一致性，并在肿瘤的进展中起一定作用。
목적：탐토위암조직중세포주기단백E적표체급기여위암림상병리인소적관계。방법：용면역조직화학방법검측료40례위암조직중세포주기단백E화p53적표체，용확증각단백19mRNA적소식RT-PCR방법검측주위정맥혈미전이。결과：40례위암조직중세포주기단백E표체양성솔위45.0%，기표체여종류침윤심도、TNM분기급외주혈미전이밀절상관(P<0.05)。p53단백표체양성솔위52.5%，재세포주기단백E양성위암중p53양성솔명현고우세포주기단백E음성위암(P<0.05)。결론：세포주기단백E재위암중적표체여p53적표체정명현적일치성，병재종류적진전중기일정작용。
Objective:To study the relationship between the expression of cyclin E and clinicopathologic factors in gastric carcinomas.Methods:Expression of cyclin E and p53 in 40 cases of gastric carcinomas were detected by immunohistochemical method,micrometastasis in peripheral blood was detected with nested RT-PCR amplification of cytokeratin 19 mRNA.Results:The total positive rate of cyclin E in 40 gastric carcinomas was 45.0%,the expression of cyclin E was significantly correlated with depth of invasion,TNM stage and micrometastasis in peripheral blood (P<0.05).The positive rate of p53 protein was 52.0%,the incidence of p53 expression was higher in cyclin E prositive tumors than that in the other tumors (P<0.05).Conclusions:These data suggest that expression of cyclin E correlates with p53 expression and may contributes to the progression of gastric carcinoma.