CAJ | 학술논문

以宁夏吴忠胡萝卜、陕西华县胡萝卜、陕西汉中胡萝卜3个地方品种为材料，用PCR方法克隆了中国胡萝卜var\%sativus\%HoffmDeutschl的抗冻蛋白基因\%afp\%，测定了宁夏吴忠胡萝卜\%afp\%的核苷酸序列，和英国胡萝卜var\%autumn\%King\%afp\%序列对比,在所测1004个核苷酸中，有35个碱基不同,其中无义突变20个，有义突变15个。按有义突变计，同源性为\{98.5%\}
이저하오충호라복、협서화현호라복、협서한중호라복3개지방품충위재료，용PCR방법극륭료중국호라복var\%sativus\%HoffmDeutschl적항동단백기인\%afp\%，측정료저하오충호라복\%afp\%적핵감산서렬，화영국호라복var\%autumn\%King\%afp\%서렬대비,재소측1004개핵감산중，유35개감기불동,기중무의돌변20개，유의돌변15개。안유의돌변계，동원성위\{98.5%\}
Antifreeze protein gene (afp) in three native carrot cultivars(Daucus carota var \%sativus\% Hoffm Deutschl),Wuzhong carrot in Ningxia,H uaxian carrot in Shaanxi and Hanzhong carrot in Shaanxi,was cloned by PCR (polym erase chain reaction).Wuzhong carrots afp was sequenced and its sequence w as compared with that of Daucus carota var \%autumn\% King from British.Ther e were 35 different bases between two varieties in 1004 sequenced nucleotides,among which there were 20 nonsense mutations and 15 sense mutations.Based on sense mutations homology was 98.5%.