肿瘤防治杂志
腫瘤防治雜誌
종류방치잡지
CHINA JOURNAL OF CANCER PREVENTION AND TREATMENT
2001年
1期
25-27
,共3页
杨崇美%张安忠%刘吉勇%王义渠
楊崇美%張安忠%劉吉勇%王義渠
양숭미%장안충%류길용%왕의거
肝肿瘤,治疗%同位素%聚合白蛋白
肝腫瘤,治療%同位素%聚閤白蛋白
간종류,치료%동위소%취합백단백
目的：研究肝癌瘤内单纯注射32p胶体和先注射聚合白蛋白(MAA)，再注射32p胶体两种不同给药方法的32p在瘤外组织器官的动态分布，探讨不同剂量MAA的阻滞效果及MAA颗粒数量和32p胶体应用剂量的相关关系。方法：在Balb/c小鼠右侧胸前皮下接种H22肝癌细胞，10 d后接种部位长出直径约1 cm的肿瘤。随机将其分为4组：第1组单纯注射32p胶体1.85 MBq；第2组先注射1×104颗粒MAA，再注射32p胶体1.85 MBq；第3组先注射1×105颗粒MAA，再注射32p胶体1.85 MBq；第4组先注射1×105颗粒MAA，再注射32p胶体18.5 MBq。注射后24 h、第8天和第16天时各组分别处死小鼠，测定心、肝、肾、脾、肺和骨骼的放射性。结果：瘤内注射32p胶体时，32p可向全身其他器官扩散；当向肿瘤内注射的32p胶体剂量相同时，预先注入1×104颗粒MAA和1×105颗粒MAA的两组小鼠，其体内32p的分布均比未注射MAA的一组小鼠要少，其中1×105颗粒MAA组的小鼠，32p体内分布又比1×104颗粒MAA组少；当预先注入的MAA颗粒数量相同时，注射的32p胶体剂量增大，体内分布也随之增加。结论：与单纯瘤内注射32p胶体相比，先在瘤内注入MAA，再注入32p胶体，MAA可以有效阻止32p胶体的全身扩散，使32p胶体能够较长时间的滞留在肿瘤内，从而减少了全身分布。
目的：研究肝癌瘤內單純註射32p膠體和先註射聚閤白蛋白(MAA)，再註射32p膠體兩種不同給藥方法的32p在瘤外組織器官的動態分佈，探討不同劑量MAA的阻滯效果及MAA顆粒數量和32p膠體應用劑量的相關關繫。方法：在Balb/c小鼠右側胸前皮下接種H22肝癌細胞，10 d後接種部位長齣直徑約1 cm的腫瘤。隨機將其分為4組：第1組單純註射32p膠體1.85 MBq；第2組先註射1×104顆粒MAA，再註射32p膠體1.85 MBq；第3組先註射1×105顆粒MAA，再註射32p膠體1.85 MBq；第4組先註射1×105顆粒MAA，再註射32p膠體18.5 MBq。註射後24 h、第8天和第16天時各組分彆處死小鼠，測定心、肝、腎、脾、肺和骨骼的放射性。結果：瘤內註射32p膠體時，32p可嚮全身其他器官擴散；噹嚮腫瘤內註射的32p膠體劑量相同時，預先註入1×104顆粒MAA和1×105顆粒MAA的兩組小鼠，其體內32p的分佈均比未註射MAA的一組小鼠要少，其中1×105顆粒MAA組的小鼠，32p體內分佈又比1×104顆粒MAA組少；噹預先註入的MAA顆粒數量相同時，註射的32p膠體劑量增大，體內分佈也隨之增加。結論：與單純瘤內註射32p膠體相比，先在瘤內註入MAA，再註入32p膠體，MAA可以有效阻止32p膠體的全身擴散，使32p膠體能夠較長時間的滯留在腫瘤內，從而減少瞭全身分佈。
목적：연구간암류내단순주사32p효체화선주사취합백단백(MAA)，재주사32p효체량충불동급약방법적32p재류외조직기관적동태분포，탐토불동제량MAA적조체효과급MAA과립수량화32p효체응용제량적상관관계。방법：재Balb/c소서우측흉전피하접충H22간암세포，10 d후접충부위장출직경약1 cm적종류。수궤장기분위4조：제1조단순주사32p효체1.85 MBq；제2조선주사1×104과립MAA，재주사32p효체1.85 MBq；제3조선주사1×105과립MAA，재주사32p효체1.85 MBq；제4조선주사1×105과립MAA，재주사32p효체18.5 MBq。주사후24 h、제8천화제16천시각조분별처사소서，측정심、간、신、비、폐화골격적방사성。결과：류내주사32p효체시，32p가향전신기타기관확산；당향종류내주사적32p효체제량상동시，예선주입1×104과립MAA화1×105과립MAA적량조소서，기체내32p적분포균비미주사MAA적일조소서요소，기중1×105과립MAA조적소서，32p체내분포우비1×104과립MAA조소；당예선주입적MAA과립수량상동시，주사적32p효체제량증대，체내분포야수지증가。결론：여단순류내주사32p효체상비，선재류내주입MAA，재주입32p효체，MAA가이유효조지32p효체적전신확산，사32p효체능구교장시간적체류재종류내，종이감소료전신분포。
Objective To study systemic distribution of colloidal 32p in single intratumoral injection and in-tratumoral injection of MAA followed by colloidal 32P, to observe retardent effect of MAA and investigate the re-lation between the quantity of MAA particles and the dose of colloidal 32P. Methods Balb/c mices were inocu-lated subcutaneously by H22 hepatocellular cancer cells in the right chest. When the tumors reach 1.0 cm of di-ameter,about 10 days postinjection,The mices were divided into four groups randomly. Group A:only injected1.85 MBq colloidal 32P; group B: 1 × 104 particles of MAA followed by 1.85 MBq colloidal 32p; group C: 1 ×105 particles of MAA followed by 1.85 MBq colloidal 32P;group D: 1 × 105 particles of MAA followed by 18.5MBq colloidal 32P.The mices of each group were killed at 24 h, 8 d and 16 d postinjection. The heart, live,spleen, kidney, lung and bone of each animal were counted for radioactivity in a gamma counter. Results 32 pmay diffuse from tumor to other organs after injecting into tumors, when the dose of 32P colloidal injected intotumor was equal, the systemic distribution of colloidal 32P in injected MAA groups in advance was lower thanthat in only injected colloidal 32P group,but the systemic distribution of 32P in 1 × 105 group was lower than thatin 1 × 104 group;when the quantity of MAA particles was equal,distribution of 32P increased following the in-crease of injected intratumoral colloidal 32P. Conclusions To inject MAA into the centre of tumors before in-ject colloidal 32P, MAA could retard diffusion of colloidal 32P from tumor tissue to the other outer-tumor organsand deposit colloidal 32P in the tumors for longer time,there for decrease the systemic distribution.
