CAJ | 학술논문

为研究在克山病病区粮食中补充蛋氨酸对大鼠组织硒和谷胱甘肽过氧化物酶(GPX)活性的影响,用克山病病区生产的低硒粮食为主配成低硒基础饲料，其硒含量为0.007mg/kg。在此基础上添加不同量的硒蛋氨酸，使饲料硒水平分别达到0.007、0.06和0.50mg/kg。每一硒水平又分别补充或不补充4g/kgDL-蛋氨酸，配制成含不同硒和蛋氨酸的6种饲料，分别喂养雄性Wistar断乳大鼠8周。结果在饲料硒水平为0.007mg/kg时，补充蛋氨酸组动物除肌肉硒含量低于未补充组外，其它组织硒含量和各组织GPX活力与不补充蛋氨酸动物无显著差异；在饲料硒水平为0.06mg/kg时，补充蛋氨酸组动物组织中的硒含量出现了重新分布，最明显的是补充蛋氨酸组动物肌肉的硒含量减少，而肝脏和血硒含量增加，且各组织中GPX活力显著大于未补充蛋氨酸组的动物；在饲料硒水平为0.50mg/kg时，补充蛋氨酸组动物组织中硒含量有不同程度下降，但GPX活力仍保持不变。研究结果认为病区粮食中蛋氨酸不足时，机体首先利用膳食中的硒蛋氨酸（谷类食物中硒的主要形式）以替代蛋氨酸参与组织蛋白质的合成。补充蛋氨酸后，硒蛋氨酸即可发挥其应有生理功能。进一步提示病区粮食中蛋氨酸不足可能是与克山病发病有关的另一因素。
위연구재극산병병구양식중보충단안산대대서조직서화곡광감태과양화물매(GPX)활성적영향,용극산병병구생산적저서양식위주배성저서기출사료，기서함량위0.007mg/kg。재차기출상첨가불동량적서단안산，사사료서수평분별체도0.007、0.06화0.50mg/kg。매일서수평우분별보충혹불보충4g/kgDL-단안산，배제성함불동서화단안산적6충사료，분별위양웅성Wistar단유대서8주。결과재사료서수평위0.007mg/kg시，보충단안산조동물제기육서함량저우미보충조외，기타조직서함량화각조직GPX활력여불보충단안산동물무현저차이；재사료서수평위0.06mg/kg시，보충단안산조동물조직중적서함량출현료중신분포，최명현적시보충단안산조동물기육적서함량감소，이간장화혈서함량증가，차각조직중GPX활력현저대우미보충단안산조적동물；재사료서수평위0.50mg/kg시，보충단안산조동물조직중서함량유불동정도하강，단GPX활력잉보지불변。연구결과인위병구양식중단안산불족시，궤체수선이용선식중적서단안산（곡류식물중서적주요형식）이체대단안산삼여조직단백질적합성。보충단안산후，서단안산즉가발휘기응유생리공능。진일보제시병구양식중단안산불족가능시여극산병발병유관적령일인소。
In order to study the effect of dietary methionine on tissueselenium and glutathione peroxidase activity(GPX) in rats fed on grains from Keshan Disease endemic area, a nonpurified selenium(Se) deficient diet(containing Se 0.007mg/kg diet)taken from Keshan Disease endemic area was supplemented with selenomethionine (SeMet) to provide selenium content in diet for 0.007,0.06 and 0.50mg/kg and added or not added with DL-methionine (Met) 4g/kg to make 6 kinds of diet. Fifty four male weanling Wistar rats were randomly divided into 6 groups, consuming each one of the 6 diets for 8 weeks. The Se content and GPX activity in tissues of animals with Met supplementation were compared with those without Met supplementation. The results showed that adding Met did not result in significant changes of tissue Se content and GPX activity in animals having dietary Se of 0.007mg/kg, except for lower muscle Se content. However, in animals having dietary Se of 0.06mg/kg and supplementing Met resulted in selenium redistribution in tissues-decrease of Se content in muscle, increase of Se content in liver and blood and significant elevation of GPX activity in all tissues. In animals having methionine-added and dietary Se of 0.50mg/kg, tissue Se content declined to various extent, while GPX activity remained unchanged. The results suggest that SeMet(main chemical form of Se in cereals) is preferentially incorporated into body protein when dietary methionine is limited. Once Met is supplemented, dietary SeMet would provide more Se for the syntheses of GPX and other selenoproteins. The results further suggest that marginal deficiency in sulfur-contaning amino acids in the diet from Keshan Disease endemic area might be an additional factor for the development of Keshan Disease under selenium deficiency.