CAJ | 학술논문

采用在茶树细胞培养过程中饲喂标记前体的方法，进行了14C标记儿茶素的合成研究。比较了14C-苯丙氨酸和14C -乙酸钠两种前体的饲喂效果，分析了饲喂前体被培养细胞吸收与利用的动态，研究了细胞预培养和紫外线预处理对提高产物标记率的作用，并采用高速逆流色谱和高效液相色谱技术对合成的标记产物进行了分析。
채용재다수세포배양과정중사위표기전체적방법，진행료14C표기인다소적합성연구。비교료14C-분병안산화14C -을산납량충전체적사위효과，분석료사위전체피배양세포흡수여이용적동태，연구료세포 예배양화자외선예처리대제고산물표기솔적작용，병채용고속역류색보화고효액상색보기술 대합성적표기산물진행료분석。
The preparation of 14C labelled catechins was studied by feeding 14C labelled precursor to tea cultured cells. Two labelled pr ecursors were tested and their effects were compared. The dynamics of absorption and transformation of fed precursors were analyzed and the effects of pre-cult ure as well as UV light pretreatment on product labelling rate were evaluated. P roduct analysis was also made by HSCCC and HPLC techniques.