第一军医大学学报
第一軍醫大學學報
제일군의대학학보
JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY
2000年
6期
513-514
,共2页
花椒油素%钙离子%钙离子载体%血小板聚集%荧光测定法
花椒油素%鈣離子%鈣離子載體%血小闆聚集%熒光測定法
화초유소%개리자%개리자재체%혈소판취집%형광측정법
xanthoxylin%calcium ion%calcimycin%platelet aggregation%cytosolic free Ca2+%fluorescence analysis
目的 研究花椒油素对CaCl2和钙离子载体A23187调节血小板及其中Ca2+浓度的作用。方法 比浊法测定血小板聚集，用Fura-2/AM荧光法测定细胞内游离钙的水平。结果 花椒油素显著地抑制CaCl2和A23187诱导的血小板聚集，前者的抑制率为9.0%~67.1%，后者的抑制率为18.1%~72.5%。明显降低A23187诱导的血小板胞质游离Ca2+浓度，降低率为12.8%~63.4%。结论 花椒油素抑制聚集作用与降低细胞内游离Ca2+水平之间呈线性正相关（P<0.01）。
目的 研究花椒油素對CaCl2和鈣離子載體A23187調節血小闆及其中Ca2+濃度的作用。方法 比濁法測定血小闆聚集，用Fura-2/AM熒光法測定細胞內遊離鈣的水平。結果 花椒油素顯著地抑製CaCl2和A23187誘導的血小闆聚集，前者的抑製率為9.0%~67.1%，後者的抑製率為18.1%~72.5%。明顯降低A23187誘導的血小闆胞質遊離Ca2+濃度，降低率為12.8%~63.4%。結論 花椒油素抑製聚集作用與降低細胞內遊離Ca2+水平之間呈線性正相關（P<0.01）。
목적 연구화초유소대CaCl2화개리자재체A23187조절혈소판급기중Ca2+농도적작용。방법 비탁법측정혈소판취집，용Fura-2/AM형광법측정세포내유리개적수평。결과 화초유소현저지억제CaCl2화A23187유도적혈소판취집，전자적억제솔위9.0%~67.1%，후자적억제솔위18.1%~72.5%。명현강저A23187유도적혈소판포질유리Ca2+농도，강저솔위12.8%~63.4%。결론 화초유소억제취집작용여강저세포내유리Ca2+수평지간정선성정상관（P<0.01）。
Objective To study the effects of xanthoxylin on platelet aggregation and freeCa2+ level induced by CaCl2 and A23187. Methods Platelet aggregation and free Ca2+ level in the platelet cytosol were measured by turbidimetry and fluorimetry of Fura-2/AM. Results Xanthoxylin at the concentration of 0.037, 0.185, 0.924, 9.240, 92.40 μmol/L significantly inhibited CaCl2 and A23187-induced platelet aggregation, with the inhibition rates ranging from 9.0% to 67.1% for CaCl2 and from 18.1% to 72.5% for A23187. A23187-induced free-Ca2+ level in the platelet cytosol was also significantly reduced (from 12.8% to 63.4%). Conclusion The inhibition rate of platelet aggregation was positively correlated with the reduction rate of cytosolic free-Ca2+ level by xanthoxylin.
