CAJ | 학술논문

目的：观察正常成人和成体大鼠气管软骨的组织动力学。方法：应用细胞生物学方法、衰老相关-β-半乳糖苷酶组织化学染色和增殖细胞核抗原免疫组织化学方法，研究正常成人和正常成体大鼠气管软骨组织动力学。结果：软骨细胞呈现多种方式的无丝分裂像；气管软骨膜与软骨周围带多见细胞增生；气管软骨中央带多见衰老的软骨细胞，并见明显核固缩、核碎裂等细胞凋亡形态学特征。结论：从软骨膜最外层致密结缔组织到成熟软骨组织呈现一个连续的组织演化动力学过程；气管软骨组织适应性再生的细胞来源是软骨膜结缔组织细胞。
목적：관찰정상성인화성체대서기관연골적조직동역학。방법：응용세포생물학방법、쇠로상관-β-반유당감매조직화학염색화증식세포핵항원면역조직화학방법，연구정상성인화정상성체대서기관연골조직동역학。결과：연골세포정현다충방식적무사분렬상；기관연골막여연골주위대다견세포증생；기관연골중앙대다견쇠로적연골세포，병견명현핵고축、핵쇄렬등세포조망형태학특정。결론：종연골막최외층치밀결체조직도성숙연골조직정현일개련속적조직연화동역학과정；기관연골조직괄응성재생적세포래원시연골막결체조직세포。
Aim: To study the histodynamics of the normal tracheal cartileage. Methods: cell biological assay, histochemical staining of senescence associated-β-galactosidase and immunohistochemical staining of proliferating cell nuclear antigen wereused. Results: chondrocytes exhibited various types of amitotic figures. The proliferating cells were mainly distributed in the peri-chondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone whereapoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis. Conclusion: The evolution fromdense connective tissue of perichondrial outmost layer to mature cartilaginous tissue showed a continuous evolving histokinetic pro-cess. The tracheal perichondrium was the originating site of adaptive cartilage regeneration.