CAJ | 학술논문

对台中65(粳稻)/Bhadua(籼稻)杂交F2代群体构建了RFLP连锁图谱,含94个分布较为均匀的标记.对F2小穗不育性状进行单点分析和区间分析的结果基本一致:有两个F2小穗不育QTL座位分别位于染色体1的XNpb113～XNpb346之间和染色体8的G187～XNpb397之间,而且该两个QTL均为新检测出的座位;检测出5个抽穗期QTL,其中3个座位在单点分析和区间分析中的结果一致,分别位于染色体1的XNpb113～XNpb346,染色体4的C891～C335,染色体8的C166～C1121,另外,染色体6的XNpb27为单点分析结果,染色体10的R716～C405为区间分析结果.由于染色体1上的F2不育QTL和抽穗期QTL重叠,该QTL座位是由于遗传效应所至还是由于环境因素(迟抽穗)所至有待构建近等基因系进一步研究.位于染色体1和10上的抽穗期QTL座位为新检测的座位.对新检测的F2不育和抽穗期QTL座位正在建立相应的近等基因系以精确定位和克隆上述基因.
대태중65(갱도)/Bhadua(선도)잡교F2대군체구건료RFLP련쇄도보,함94개분포교위균균적표기.대F2소수불육성상진행단점분석화구간분석적결과기본일치:유량개F2소수불육QTL좌위분별위우염색체1적XNpb113～XNpb346지간화염색체8적G187～XNpb397지간,이차해량개QTL균위신검측출적좌위;검측출5개추수기QTL,기중3개좌위재단점분석화구간분석중적결과일치,분별위우염색체1적XNpb113～XNpb346,염색체4적C891～C335,염색체8적C166～C1121,령외,염색체6적XNpb27위단점분석결과,염색체10적R716～C405위구간분석결과.유우염색체1상적F2불육QTL화추수기QTL중첩,해QTL좌위시유우유전효응소지환시유우배경인소(지추수)소지유대구건근등기인계진일보연구.위우염색체1화10상적추수기QTL좌위위신검측적좌위.대신검측적F2불육화추수기QTL좌위정재건립상응적근등기인계이정학정위화극륭상술기인.
Ninety-six F2 lines derived from a cross between a japonica cultivar Taichung 65 and an indica cultivar Bhadua were developed. At the first step, an RFLP linkage map based on the F2 lines was constructed. The RFLP map contained 94 RFLP makers. F2 sterility and heading date are important agronomic traits of rice; meanwhile heading date is related to many characters of agronomic importance including sterility. Quantitative trait locus (QTL) analysis was carried out to identify genes controlling F2 sterility and heading date. Both single factor analysis and interval analysis were applied for QTL analysis. Two QTLs for F2 spikelet sterility were newly detected on Chromosome 1 and 8. Five QTLs for heading date were detected on Chromosome 1, 4, 6,8 and 10. Two of them on chromosome1 and 10 were newly detected. Near-isogenic lines are now under construction for further QTL analysis and gene mapping of these QTLs newly identified in this paper.