CAJ | 학술논문

以纤维素膜为基质材料,制备了3种用于去除内毒素的亲和膜,分别为壳聚糖亲和膜(KFCC517),疏水阳离子亲和膜(KFCG316)和金属螯合物亲和膜(KFCG402).研究了亲和膜对内毒素的吸附容量及在内毒素去除方面的应用,结果表明这3种亲和膜都可以用于多种溶液中内毒素的去除.考察了离子强度、pH值以及流速对去除效果的影响,并分析了原因.KFCC517,KFCG316,KFCG402都可用于人血清白蛋白溶液中内毒素的去除,KFCC517还可用于医药制剂如氢化可的松、葛根素、盐酸丁卡因和右旋糖苷40葡萄糖注射液中内毒素的去除,在所选择的条件下,内毒素的去除效率高于80%,有用物质的回收率为95%以上.
이섬유소막위기질재료,제비료3충용우거제내독소적친화막,분별위각취당친화막(KFCC517),소수양리자친화막(KFCG316)화금속오합물친화막(KFCG402).연구료친화막대내독소적흡부용량급재내독소거제방면적응용,결과표명저3충친화막도가이용우다충용액중내독소적거제.고찰료리자강도、pH치이급류속대거제효과적영향,병분석료원인.KFCC517,KFCG316,KFCG402도가용우인혈청백단백용액중내독소적거제,KFCC517환가용우의약제제여경화가적송、갈근소、염산정잡인화우선당감40포도당주사액중내독소적거제,재소선택적조건하,내독소적거제효솔고우80%,유용물질적회수솔위95%이상.
Three kinds of affinity membranes utilized for endotoxin removal, namely, chitosan affinity membrane (KFCC517), hydrophobic and cationic charged membrane (KFCG316) and metal chelate affinity membrane (KFCM402) were prepared by using cellulose membrane as support. The adsorbability and applications of the membranes in endotoxin removal were investigated. The results showed that all of these affinity membranes could be utilized for endotoxin removal in several kinds of solutions. The factors affecting endotoxin removal such as ionic strength, pH value and flow rate were investigated, and the results were discussed. Three cartridges of membranes KFCC517, KFCG316 and KFCM402 were utilized for endotoxin removal in human serum albumin solution. The cartridge of membrane KFCC517 also was utilized in medicine injections, such as hydrocortisone, puerarin, tetracaine hydrochloride and dextran 40 glucose. Under the optimal conditions, the removal efficiencies of endotoxin were over 80% and the recoveries of desirable substances were over 95%.