CAJ | 학술논문

采用液相色谱/质谱/质谱法，通过保留时间、分子量和二级质谱的信息对复方“清开灵”注射液中的胆酸、去氧胆酸和鹅去氧胆酸进行了定性；建立了内标法对复方中相对含量较大的胆酸的定量分析方法；结果表明胆酸在875 ng/L～140 μg/L范围内线性良好，线性相关系数R2=0.9999；RSD=2.4%。该方法样品处理简单，选择性好，灵敏度高。对中药的质量控制具有重要的意义。
채용액상색보/질보/질보법，통과보류시간、분자량화이급질보적신식대복방“청개령”주사액중적담산、거양담산화아거양담산진행료정성；건립료내표법대복방중상대함량교대적담산적정량분석방법；결과표명담산재875 ng/L～140 μg/L범위내선성량호，선성상관계수R2=0.9999；RSD=2.4%。해방법양품처리간단，선택성호，령민도고。대중약적질량공제구유중요적의의。
By comparing with the molecule weights, retention times and product ion spectra of the standards, the cholic acid, deoxycholic acid and chenodeoxycholic acid were identified in the traditional Chinese compound medicine——“Qingkailing” injection by high performance liquid chromatography/mass spectrometry/mass spectrometry. Two other isomers of cholic acid and deoxycholic acid were also found, but tieir detail structure was still unknown due to the absence of standards. A quantitative method to determine cholic acid in the medicine was established and studied. An internal standard was used in quantitative analysis. When the concentration of cholic acid is from 875 ng/L to 140 μg/L, the chromatographic peak area and concentration are of good linear relation. The linear correlation coefficient was determined to be 0.9999 and the RSD is 2.04%. This method is simple, selective and highly sensitive, which is helpful for the quality control of traditional Chinese medicine.