细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2001年
4期
389-391
,共3页
陈晓东%吴伯瑜%黄逢元%江琼
陳曉東%吳伯瑜%黃逢元%江瓊
진효동%오백유%황봉원%강경
烧伤%红细胞免疫粘附功能%氧自由基
燒傷%紅細胞免疫粘附功能%氧自由基
소상%홍세포면역점부공능%양자유기
目的探讨氧自由基对烧伤后红细胞免疫粘附功能的影响。方法于伤后 1 d、 3 d、 5 d、 7 d、 14 d、 21 d、 28 d和 35d,观察 RBC-C3b受体花环形成率 (RC3b RR)和 RBC-IC花环形成率 (RICR),以及 SOD、 Lpo-MDA、 CAT、 GSH-px和 GSH水平的变化,并进行相关性分析。结果①伤后 RC3bRR显著下降, RICR显著升高 (P< 0.05~ 0.001),但 4wk后逐渐恢复至正常。②伤后 SOD、 CAT、 GSH-px和 GSH的水平显著下降, Lpo-MDA的水平则显著上升 (P< 0.05~ 0.001),至少伤后 3wk才恢复到正常水平。③ RC3bRR与 SOD、 CAT、 GSH-px和 GSH的水平呈显著的正相关 (r值依次分别为 :0.601, 0.799, 0.715和 0.597),与 Lpo-MDA的水平呈显著的负相关 (r=-0.564); RICR与 SOD、 CAT、 GSH-px和 GSH的水平也呈显著的负相关 (r值依次分别为 :-0.359、 -0.577、 -0.420和 -0.427),与 Lpo-MDA的水平则呈显著的正相关 (r=0.433)。结论红细胞膜极易产生脂质过氧化作用,烧伤后自由基损伤的程度,可直接影响红细胞膜的功能,特别是红细胞免疫粘附功能。在烧伤治疗过程中,提示减少机体自由基的产生,提高患者红细胞免疫粘附功能是十分必要的。
目的探討氧自由基對燒傷後紅細胞免疫粘附功能的影響。方法于傷後 1 d、 3 d、 5 d、 7 d、 14 d、 21 d、 28 d和 35d,觀察 RBC-C3b受體花環形成率 (RC3b RR)和 RBC-IC花環形成率 (RICR),以及 SOD、 Lpo-MDA、 CAT、 GSH-px和 GSH水平的變化,併進行相關性分析。結果①傷後 RC3bRR顯著下降, RICR顯著升高 (P< 0.05~ 0.001),但 4wk後逐漸恢複至正常。②傷後 SOD、 CAT、 GSH-px和 GSH的水平顯著下降, Lpo-MDA的水平則顯著上升 (P< 0.05~ 0.001),至少傷後 3wk纔恢複到正常水平。③ RC3bRR與 SOD、 CAT、 GSH-px和 GSH的水平呈顯著的正相關 (r值依次分彆為 :0.601, 0.799, 0.715和 0.597),與 Lpo-MDA的水平呈顯著的負相關 (r=-0.564); RICR與 SOD、 CAT、 GSH-px和 GSH的水平也呈顯著的負相關 (r值依次分彆為 :-0.359、 -0.577、 -0.420和 -0.427),與 Lpo-MDA的水平則呈顯著的正相關 (r=0.433)。結論紅細胞膜極易產生脂質過氧化作用,燒傷後自由基損傷的程度,可直接影響紅細胞膜的功能,特彆是紅細胞免疫粘附功能。在燒傷治療過程中,提示減少機體自由基的產生,提高患者紅細胞免疫粘附功能是十分必要的。
목적탐토양자유기대소상후홍세포면역점부공능적영향。방법우상후 1 d、 3 d、 5 d、 7 d、 14 d、 21 d、 28 d화 35d,관찰 RBC-C3b수체화배형성솔 (RC3b RR)화 RBC-IC화배형성솔 (RICR),이급 SOD、 Lpo-MDA、 CAT、 GSH-px화 GSH수평적변화,병진행상관성분석。결과①상후 RC3bRR현저하강, RICR현저승고 (P< 0.05~ 0.001),단 4wk후축점회복지정상。②상후 SOD、 CAT、 GSH-px화 GSH적수평현저하강, Lpo-MDA적수평칙현저상승 (P< 0.05~ 0.001),지소상후 3wk재회복도정상수평。③ RC3bRR여 SOD、 CAT、 GSH-px화 GSH적수평정현저적정상관 (r치의차분별위 :0.601, 0.799, 0.715화 0.597),여 Lpo-MDA적수평정현저적부상관 (r=-0.564); RICR여 SOD、 CAT、 GSH-px화 GSH적수평야정현저적부상관 (r치의차분별위 :-0.359、 -0.577、 -0.420화 -0.427),여 Lpo-MDA적수평칙정현저적정상관 (r=0.433)。결론홍세포막겁역산생지질과양화작용,소상후자유기손상적정도,가직접영향홍세포막적공능,특별시홍세포면역점부공능。재소상치료과정중,제시감소궤체자유기적산생,제고환자홍세포면역점부공능시십분필요적。
Aim To investigate the influence of oxygen-free radical to erythrocyte immune adherent function after severe burns. Methods The erythrocyte's immune adherent function (RC3bRR and RICR) and the levels of SOD, Lpo-MDA, CAT, GSH-px and GSH were detected on 1, 3, 5, 7, 14, 21, 28 and 35 days after severe burns, and then their correlations were analyzed. Results The rosette forming rate of erythrocyte C3b receptor (RC3bRR) in post-burn group was obviously lower than that in normal control group (P< 0.05~ 0.001), and erythrocyte immune complex rosette forming rate (RICR) of the burned patients was obviously higher than that of the healthy group(P< 0.001), but they gradually returned to normal four weeks after burn. Compared with healthy control group, the levels of SOD, CAT, GSH-px and GSH markedly declined(P< 0.05~ 0.001), while Lpo-MDA level notably increased(P< 0.05~ 0.001), but their recovery needed at least three weeks. RC3bRR was positively correlated with the levels of SOD, CAT, GSH-px and GSH, and was negatively correlated with Lpo-MDA. Correlation of RICR was contrary to that of RC3bRR. Conclusion The degree of the free radical injury may directly influence the erythrocyte membrane function, especially the erythrocyte immune adherent. It is necessary to reducing the production of free radicals and enhacing patient's erythrocyte immune function during burn treatment.