CAJ | 학술논문

[目的]研究花生四烯酸高产突变株的发酵及菌丝老化特性.[方法]对1株经诱变获得的花生四烯酸高产突变株 A1.13进行5 L罐发酵试验,考察不同培养条件对发酵产物的影响.[结果]碳源一次性过量加入并改变培养温度,可使该菌株花生四烯酸产量提高至602.99 mg/L;发酵菌丝体在4 ℃条件下老化15 d后,花生四烯酸产量达885.16 mg/L.大豆油、花生油、玉米油、葵花籽油和芝麻油5种植物油均可起到消泡作用,添加浓度4%葵花籽油效果最佳,与对照相比,菌丝生物量和花生四烯酸产量分别提高了124%和22%.[结论]该研究可为突变株 A1.13工业化发酵产花生四烯酸提供理论依据和现实参考.
[목적]연구화생사희산고산돌변주적발효급균사노화특성.[방법]대1주경유변획득적화생사희산고산돌변주 A1.13진행5 L관발효시험,고찰불동배양조건대발효산물적영향.[결과]탄원일차성과량가입병개변배양온도,가사해균주화생사희산산량제고지602.99 mg/L;발효균사체재4 ℃조건하노화15 d후,화생사희산산량체885.16 mg/L.대두유、화생유、옥미유、규화자유화지마유5충식물유균가기도소포작용,첨가농도4%규화자유효과최가,여대조상비,균사생물량화화생사희산산량분별제고료124%화22%.[결론]해연구가위돌변주 A1.13공업화발효산화생사희산제공이론의거화현실삼고.
[Objective]The research aimed to study the fermentation of high arachidonic acid-producing mutant and the mycelium aging.[Method]Fermentation of high arachidonic acid-producing mutant A1.13 was taken in 5 L fermenter.And the products under different culture conditions was investigated.[Result]The results showed that arachidonic acid (AA) yield could reach 602.99 mg/L in the batch fermentation process when large amount of carbon source was added and culture temperature were altered.After aging 15 d at 4 ℃, AA yield reached 885.16 mg/L.Five kinds of plant oils (soybean oil, arachis oil, maize oil, sunflower seed oil, sesame oil) could all be used as antifoam.Compared with the CK treatment,concentration 4% sunflower seed oil significantly increased the biomass and the AA yield by 124% and 22% respectively.[Conclusion]This study could provide theoretical basis and realistic reference for the production of AA with mutant A1.13 by industrial fermentation.