食品科学
食品科學
식품과학
FOOD SCIENCE
2009年
23期
294-296
,共3页
新疆圆柏%内生菌%分离%抑菌活性
新疆圓柏%內生菌%分離%抑菌活性
신강원백%내생균%분리%억균활성
Sabina vulgaris Ant%endophytic bacteria%isolation%antibacterial activity
对新疆圆柏叶内生菌进行分离,确定分离条件,分离纯化得到7株菌株,采用打孔法对这7株菌的发酵液进行抑菌实验.结果表明:其中两株菌的发酵液对枯草芽孢杆菌、金黄色葡萄球菌呈现较强的抑菌活性.通过形态学观察和革兰氏染色,确定1号菌株为革兰氏阳性球菌,2号菌株为革兰氏阴性球菌,均无荚膜,无芽孢,不产酸,不产气,淀粉水解为阴性.
對新疆圓柏葉內生菌進行分離,確定分離條件,分離純化得到7株菌株,採用打孔法對這7株菌的髮酵液進行抑菌實驗.結果錶明:其中兩株菌的髮酵液對枯草芽孢桿菌、金黃色葡萄毬菌呈現較彊的抑菌活性.通過形態學觀察和革蘭氏染色,確定1號菌株為革蘭氏暘性毬菌,2號菌株為革蘭氏陰性毬菌,均無莢膜,無芽孢,不產痠,不產氣,澱粉水解為陰性.
대신강원백협내생균진행분리,학정분리조건,분리순화득도7주균주,채용타공법대저7주균적발효액진행억균실험.결과표명:기중량주균적발효액대고초아포간균、금황색포도구균정현교강적억균활성.통과형태학관찰화혁란씨염색,학정1호균주위혁란씨양성구균,2호균주위혁란씨음성구균,균무협막,무아포,불산산,불산기,정분수해위음성.
Seven bacteria strains were isolated form leaves of Sabina vulgaris Ant, and antibacterial activity of their fermentation products were evaluated according to the size of their anitbacterial circles. Totally 2 endophytic bacterium exhibited antibacterial activity, which could inhibit the growth of Bacillus subtilis and Staphylococcus aureus. Colonial morphology observation and Gram staining of the 2 endophytic bacterium showed that one bacteria was coccus shaped, gram-positive, and the other was coccus shaped and gram-negative, both without capsule and spore, and without producing acid and amylase.
