CAJ | 학술논문

运用蚕豆根尖细胞微核试验技术对浊漳河南段6个代表性样点水体中有机污染物的遗传毒性进行了研究,为浊漳河流域水体污染状况的有效监测提供理论依据.结果显示:(1)浊漳河南段各监测断面的水体有机污染物对蚕豆根尖细胞均产生了不同程度的损伤,表现出细胞微核、断片、染色体桥等多种异常形态,严重时导致细胞坏死和细胞凋亡.(2)6个样点水样处理的蚕豆根尖细胞微核率在21.47‰～64.77‰,极显著高于对照组(P<0.01).(3)各样点水样对蚕豆根尖细胞的遗传损伤程度依次为:王桥>店上>漳泽水库>北寨>黄碾>五阳;在有丝分裂后期,蚕豆根尖中异常细胞最高达70%,且异常细胞比率有随着污染程度增大而升高的趋势.研究表明,蚕豆根尖细胞微核是水质毒理检测的有效指标,其细胞有丝分裂后期异常细胞比率也可作为水质毒理检测的观察指标.
운용잠두근첨세포미핵시험기술대탁장하남단6개대표성양점수체중유궤오염물적유전독성진행료연구,위탁장하류역수체오염상황적유효감측제공이론의거.결과현시:(1)탁장하남단각감측단면적수체유궤오염물대잠두근첨세포균산생료불동정도적손상,표현출세포미핵、단편、염색체교등다충이상형태,엄중시도치세포배사화세포조망.(2)6개양점수양처리적잠두근첨세포미핵솔재21.47‰～64.77‰,겁현저고우대조조(P<0.01).(3)각양점수양대잠두근첨세포적유전손상정도의차위:왕교>점상>장택수고>북채>황년>오양;재유사분렬후기,잠두근첨중이상세포최고체70%,차이상세포비솔유수착오염정도증대이승고적추세.연구표명,잠두근첨세포미핵시수질독리검측적유효지표,기세포유사분렬후기이상세포비솔야가작위수질독리검측적관찰지표.
Genotoxicity of organic pollutants in the southern water body at six typical monitor section of Zhuozhang River were studied by the Vicia faba root-tip cell micronucleus technique.The results showed that:V.faba root tip cells had different degree damage by organic pollutants,including micronuclei producing,chromosomal fragments,chromosomal bridges,etc.even to cell necrosis and the cell perishes.Frequency of micronucleus of six treatments ranged from 21.47‰ to 64.77‰ and the difference was significant (P<0.01) compared with control group.The degrees of the genetic damage induced by pollutants in V.faba root tips was in sequence of Wangqiao>Dianshang>Zhangze reservoir>Beizhai>Huangnian>Wuyang.At anaphase of mitosis,the ratio of abnormal cells of root tips is a maximal of 70%,which was positively correlated with pollution degree.All of these results provide an effective basis for widespread application of V.faba root tip micronucleus assay in monitoring genotoxicity of water environment aspect.Data obtained from this study indicate that the micronuclei is an effective index for detecting water quality,and the ratio of abnormal cell at anaphase of mitosis is also as an effective observation index.