CAJ | 학술논문

目的:复制小鼠非酒精性脂肪肝动物模型,观察木瓜对此模型的影响.方法:高脂饮食同时皮下注射小剂量四氯化碳复制小鼠非酒精性脂肪肝模型,在高脂饮食中添加木瓜对其进行干预,与正常饮食组对照.喂养5周后柃测血清肝脏酶学(ALT、AST)、血脂(TG、CHO)水平;肝组织切片染色,显微镜观察肝组织病理变化;提取肝脏总RNA检测TLR-2、TLR-4、IL-1β、TGF-β、PD-L1等基因的表达情况,并进行统计学分析.结果:与正常组相比,模型组血清的肝功能和脂质水平上升,肝细胞形态明显改变,肝索消失或不清晰,相应基因表达水平上调;与模型组相比,木瓜干预组血清肝功能指标和脂质水平均显著下降,肝细胞气球样变较少,无明显肝细胞坏死,肝索存在,肝组织TLR和炎性因子表达水甲明显下调. 结论:木瓜对高脂饮食诱导的小鼠非酒精性脂肪肝有一定预防效果,该效应的发挥可能是通过调节TLR受体及死亡配受体表达,炎性因子的分泌而实现的.
목적:복제소서비주정성지방간동물모형,관찰목과대차모형적영향.방법:고지음식동시피하주사소제량사록화탄복제소서비주정성지방간모형,재고지음식중첨가목과대기진행간예,여정상음식조대조.위양5주후령측혈청간장매학(ALT、AST)、혈지(TG、CHO)수평;간조직절편염색,현미경관찰간조직병리변화;제취간장총RNA검측TLR-2、TLR-4、IL-1β、TGF-β、PD-L1등기인적표체정황,병진행통계학분석.결과:여정상조상비,모형조혈청적간공능화지질수평상승,간세포형태명현개변,간색소실혹불청석,상응기인표체수평상조;여모형조상비,목과간예조혈청간공능지표화지질수평균현저하강,간세포기구양변교소,무명현간세포배사,간색존재,간조직TLR화염성인자표체수갑명현하조. 결론:목과대고지음식유도적소서비주정성지방간유일정예방효과,해효응적발휘가능시통과조절TLR수체급사망배수체표체,염성인자적분비이실현적.
Objective:To investigate the preventive effect of Chaenomeles fruits on non-alcoholic steatohepatitis(NASH) in mice.Methods:NASH model was established by high fat diet feeding combined with hypodermic injection at low dose of CCl4.NASH model group fed high fat diet containing Chaenomeles fruits for 5 consecutive weeks was served as Chaenomeles fruitsintervened group,while the mice in control group were fed normal diet.At the end of experiments,all the mice were sacrificed to analyze ALT.AST,TG and CHO levels in serum. Pathological changes of hepatic tissues were observed under microscope and mRNA expression of genes such as TGF-β,PD-L1,TLR-2,TLR-4 and IL-1β in hepatic tissues was detected by the real time RT-PCR technique.Results:Compared to normal mice,ALT,TG and CHO levels in serum exhibited an obvious increase in NASH mice and a significant reduction in Chaenomeles fruits-intervened NASH mice(P＜0.05).Hepatocyte swelling,as well as neutrophilic infiltration and hepatocyte ballooning exhibited a decrease in Chaenomeles fruits-intervened NASH mice.Meanwhile,a significant decrease in hepatic TLR-2,TLR.4,IL-1β,TGF-β and PD-L1 were also observed in Chaenomeles fruits-intervened NASH mice.Conclusion:Chaenomeles fruits have a significant preventive effeet on non-alcoholic steatohepatifis in mice,which is achieved by regulating TLR and death receptor expression and the secretion of inflammatory cytokines.