CAJ | 학술논문

以转基因鲤和非转基因鲤鱼肉(受试物)作为大鼠的铡料基础蛋白成分,从毒性方面开展转基因鲤的食用安全检测分析.急性经口毒性检测结果表明,实验期间,受试动物未见明显的中毒表现,无死亡,尸检中主要脏器办未见异常,说明转大麻哈鱼生长激素基因鲤食用尤毒.将转基因鲤以不同质量分数(10%、5%、2.5%)掺入饲料喂饲大鼠90d,检测结果表明,受试动物活动自如,被毛有光泽,鼻、眼、口腔无异常分泌物.一般毒性检测指标与阴性对照组比较,无显著性差异;末见该受试物对大鼠的血液学指标、血生化指标、尿常规和生化指标、脏器系数、病理组织学指标有不良影响.据此评价转大麻哈鱼生长激素基因鲤在大鼠实验阶段属无毒.
이전기인리화비전기인리어육(수시물)작위대서적찰료기출단백성분,종독성방면개전전기인리적식용안전검측분석.급성경구독성검측결과표명,실험기간,수시동물미견명현적중독표현,무사망,시검중주요장기판미견이상,설명전대마합어생장격소기인리식용우독.장전기인리이불동질량분수(10%、5%、2.5%)참입사료위사대서90d,검측결과표명,수시동물활동자여,피모유광택,비、안、구강무이상분비물.일반독성검측지표여음성대조조비교,무현저성차이;말견해수시물대대서적혈액학지표、혈생화지표、뇨상규화생화지표、장기계수、병리조직학지표유불량영향.거차평개전대마합어생장격소기인리재대서실험계단속무독.
In order to investigate the toxicity of transgenic carp with salmon growth hormone gene, mice were fed diet containing flesh of transgenic carp with salmon growth hormone gene or non-transgenic carp. The.diets containing flesh of transgenic carp with different proportions of 10%, 5% and 2.5% were given to mice for 90 consecutive days. Acute oral toxicity experimental results revealed that mice fed wansgenic carp were normal as the control group fed non-transgenic carp during the period of 90 days. The mice exhibited free movements and shining coats without any abnormal symptoms and abnormal secretions in their noses, eyes and mouths. Meanwhile, no obvious difference in toxic indices between experi-mental group and control group was observed. Moreover, no abnormal change in hematological and biochemical indices, routine urine test, viscera coefficients and histopathological examinations was observed in mice fed transgenic carp with salmon growth hormone gene. Therefore, salmon growth hormone gene has no toxicity and transgenic carps are safe to mice as edible foods.