CAJ | 학술논문

综述了基因芯片在卷烟危害性评价中的应用,基因芯片可以从全基因组转录水平分析卷烟烟气对基因表达的影响,并可通过差异表达基因寻求生物标志物.卷烟烟气导致差异表达基因主要属于: 氧化应激反应类、异源物代谢类、炎症/免疫反应类、基质降解类.并且,基因芯片可从差异表达基因角度区分不同商品卷烟.评价卷烟危害性,基因芯片存在优势及不足,基因芯片结合其它生物学技术将在卷烟危害性评价发挥重要作用.
종술료기인심편재권연위해성평개중적응용,기인심편가이종전기인조전록수평분석권연연기대기인표체적영향,병가통과차이표체기인심구생물표지물.권연연기도치차이표체기인주요속우: 양화응격반응류、이원물대사류、염증/면역반응류、기질강해류.병차,기인심편가종차이표체기인각도구분불동상품권연.평개권연위해성,기인심편존재우세급불족,기인심편결합기타생물학기술장재권연위해성평개발휘중요작용.
Application of DNA microarray technologies to cigarette smoke risk evaluation was reviewed. With DNA microarray technologies, the effects of cigarette smoke on gene expression were comprehensively studied and the biomarker of human exposing to smoke was identified. Cigarette smoke-induced differentially expressed genes can be divided into different groups: oxidative stress response, xenobiotic metabolism, inflammation/immune response, and matrix degradation. In view of differential expressed genes, microarray can preferably distinguish different commercially cigarettes. Both advantage and disadvantage exist in microarray, and if combined with other molecular biological technologies, DNA microarray technologies will play an important role in cigarette smoke risk evaluation.