CAJ | 학술논문

目的观察哮喘患者肺部γδT淋巴细胞数量、分类、增殖率、所分泌的细胞因子和T细胞受体δ链的多态性,探讨肺部γδT细胞对哮喘患者气道炎症的影响.方法 7例哮喘患者和7例对照者的支气管肺泡灌洗液细胞分类计数,流式细胞术检测γδT细胞,免疫磁珠分选技术纯化γδT细胞,MTT法检测γδT细胞的增殖活性,ELISA法检测γδT细胞分泌细胞因子浓度,RT-PCR技术和基因扫描技术测定γδT细胞δ链的克隆性.结果哮喘患者组支气管肺泡灌洗液中γδT细胞的百分率为(6.39±0.71)%,高于对照组的(2.62±0.37)%(P<0.01);巨噬细胞为(81±4)%,低于对照组的(86±2)%(P<0.05);哮喘组γδT细胞的增殖率为(284.2±43.6)%,高于对照组的(217±5±59.5)%(P<0.05).γδT细胞分泌的白细胞介素-4浓度为(18.9±3.1)pg/ml,高于对照组的(14.1±3.0)pg/ml(P<0.05).γδT细胞受体δ1链、δ2链两组差异无显著性.结论 γδT细胞在支气管哮喘患者的肺部明显增多,加剧了Thl/Th2失衡.γδT细胞对抗原的识别是非特异的.
목적 관찰효천환자폐부γδT림파세포수량、분류、증식솔、소분비적세포인자화T세포수체δ련적다태성,탐토폐부γδT세포대효천환자기도염증적영향.방법 7례효천환자화7례대조자적지기관폐포관세액세포분류계수,류식세포술검측γδT세포,면역자주분선기술순화γδT세포,MTT법검측γδT세포적증식활성,ELISA법검측γδT세포분비세포인자농도,RT-PCR기술화기인소묘기술측정γδT세포δ련적극륭성.결과 효천환자조지기관폐포관세액중γδT세포적백분솔위(6.39±0.71)%,고우대조조적(2.62±0.37)%(P<0.01);거서세포위(81±4)%,저우대조조적(86±2)%(P<0.05);효천조γδT세포적증식솔위(284.2±43.6)%,고우대조조적(217±5±59.5)%(P<0.05).γδT세포분비적백세포개소-4농도위(18.9±3.1)pg/ml,고우대조조적(14.1±3.0)pg/ml(P<0.05).γδT세포수체δ1련、δ2련량조차이무현저성.결론 γδT세포재지기관효천환자적폐부명현증다,가극료Thl/Th2실형.γδT세포대항원적식별시비특이적.
ObjectiveTo observe the function of γδ T lymphocytes and the polymorphism of T cell re-ceptor γδ chain in the lungs of asthmatic patients and explore the role of γδ T cells in airway inflammation. MethodsBronchoalveolar lavage fluid ( BALF) was obtained from 7 asthmatic patients and 7 healthy con-trol individuals. The percentage of γδT cell in BALF was measured by flow cytometry. The γδT cell in BALF was purified by magnetic labeled beads. Proliferous activity was examined by MTT assay. Cytokines secreted by γδT cells in medium was assessed by enzyme-linked immunosorbent assay. Polymorphism of T cell receptor γδ chain was detected by RT-PCR and genescan analysis. Results The proportion of γδT cell in the BALF of asthmatic patients [( 6. 39 ± 0. 71 ) %] was significantly higher than that in control subjects [( 2. 62 ±0. 37 ) %] ( P < 0. 01). The proportion of macrophage in the BALF of asthmatic patients [( 81 ± 4 ) %] was significantly lower than that in control subjects [(86 ±2)%] (P < 0. 05 ). The proliferation rate of asthmatic patients [(284. 2 ±43. 6)%] was significantly higher than that of control subjects [(217. 5 ±59. 5)%] (P < 0. 05). Interleukin-4 secreted by γδT cells of asthmatic patientts [(18. 9±3. 1) pg/ml)] significantly in-creased when compared with the control subjects [( 14. 1 ± 3. 0) pg/ml] ( P < 0. 05 ). The polymorphism of T cell receptor γδ chain was not significantly different between these two groups. Conclusions The increase of γδT cells in the lung of asthmatic patients further exacerbates Th1,/Th2 disturbance and airway inflammation. Antigen recognition by γδT cells is non-specific.