CAJ | 학술논문

目的探讨支气管肺泡灌洗液(BALF)中白细胞介素(IL)-6、IL-8与小儿细菌性肺炎的相关性.方法采用双抗体夹心ELISA法分别测定26例细菌性肺炎患儿(肺炎组)治疗前后和14例无明显气道炎性反应患儿(对照组)BALF中IL-6、IL-8水平,并行中性粒细胞(PMN)和肺泡巨噬细胞(AMs)计数.结果肺炎组治疗前BALF中IL-6和IL-8水平、细胞总数、PMN明显比治疗后及对照组高(P<0.01).革兰阴性细菌感染患儿BALF中IL-6、IL-8水平比革兰阳性细菌感染显著升高(P<0.01).肺炎组BALF中IL-6水平与AMs呈显著正相关(r=0.7615.P<0.01),肺炎组BALF中IL-8水平分别与PMN和AMs呈显著正相关(r=0.8956,r=0.6018,P<0.01).结论 IL-6、IL-8参与小儿细菌性肺炎的炎性反应,BALF中IL-6和IL-8水平动态变化对评价小儿细菌性肺炎的病情有一定的临床意义.
목적 탐토지기관폐포관세액(BALF)중백세포개소(IL)-6、IL-8여소인세균성폐염적상관성.방법 채용쌍항체협심ELISA법분별측정26례세균성폐염환인(폐염조)치료전후화14례무명현기도염성반응환인(대조조)BALF중IL-6、IL-8수평,병행중성립세포(PMN)화폐포거서세포(AMs)계수.결과 폐염조치료전BALF중IL-6화IL-8수평、세포총수、PMN명현비치료후급대조조고(P<0.01).혁란음성세균감염환인BALF중IL-6、IL-8수평비혁란양성세균감염현저승고(P<0.01).폐염조BALF중IL-6수평여AMs정현저정상관(r=0.7615.P<0.01),폐염조BALF중IL-8수평분별여PMN화AMs정현저정상관(r=0.8956,r=0.6018,P<0.01).결론 IL-6、IL-8삼여소인세균성폐염적염성반응,BALF중IL-6화IL-8수평동태변화대평개소인세균성폐염적병정유일정적림상의의.
Objective To investigate the relationship between bronchoalveolar lavage fluid (BALF) levels of interleukin (IL)-6, IL-8 and child bacterial pneumonia. Methods BALF levels of IL-6, IL-8 were detected by enzyme linked immunosorbent assay (ELISA) in 26 patients with bacterial pneumonia and 14 controls. Neutrophils and alveolar macrophages (AMs) of BALF were examined. Results BALF levels of IL-6, IL-8, total cell count and neutrophils of child bacterial pneumonia before treatment were significantly higher than those of pneumonia after treatment and controls (P<0.01). BALF levels of IL-6, IL-8 were significantly higher in Gram-negative infection than those in Gram-positive infection (P<0.01). BALF level of IL-6 showed positive correlation with AMs in pneumonia (r=0.7615, P<0.01). BALF level of IL-8 was correlated positively with neutrophils and AMs respectively in pneumonia (r=0.8956, r=0.6018, P<0.01). Conclusions IL-6 and IL-8 play the roles in the development of airway inflammation in child bacterial pneumonia. Detection of BALF levels of IL-6 and IL-8 is valuable to predict the state of child bacterial pneumonia.