中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2012年
7期
581-586
,共6页
包伟晶%林丛%李志春%林昶%吴敏霞%钟秀容
包偉晶%林叢%李誌春%林昶%吳敏霞%鐘秀容
포위정%림총%리지춘%림창%오민하%종수용
催化作用%钛%鼻咽肿瘤%纳米技术%半胱氨酸天冬氨酸蛋白酶3%细胞凋亡
催化作用%鈦%鼻嚥腫瘤%納米技術%半胱氨痠天鼕氨痠蛋白酶3%細胞凋亡
최화작용%태%비인종류%납미기술%반광안산천동안산단백매3%세포조망
Catalysis%Titanium%Nasopharyngeal neoplasms%Nanotechmology%Disease models,animal%Caspase 3%Apoptosis
目的 研究光催化纳米二氧化钛(TiO2)对人鼻咽癌裸鼠移植瘤模型的治疗效果,初步探讨其抑癌机制.方法 建立荷人鼻咽癌裸鼠移植瘤模型,随机分成6组,包括实验组:3个梯度含量的纳米TiO2+紫外光照射组；阴性对照组:单纯纳米TiO2组与单纯紫外光照射组；空白对照组.其中纳米TiO2悬液采用瘤内注射,经波长范围330 ～400nm的紫外光处理后24h,取各组瘤组织HE染色作病理分析；透射电镜观察肿瘤细胞超微结构的变化及凋亡情况；采用免疫组化检测肿瘤组织Caspase-3的表达,采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法(TUNEL)检测细胞凋亡情况,并分析两者的相关性.结果 病理学分析,纳米TiO2光催化治疗后移植瘤组织的局部炎性反应明显(Ⅱ、Ⅲ级),伴随炎性反应的是局部坏死灶形成；而阴性及空白对照组无明显炎性反应(Ⅰ级).透射电镜下观察,实验组可见纳米TiO2颗粒广泛进入胞质胞核,肿瘤细胞多有凋亡的形态学改变.实验组Caspase-3的阳性表达及TUNEL标记的阳性细胞数显著多于对照组(P＜0.01),且阳性表达随TiO2含量递增而增强(P＜0.01).结论 光催化纳米TiO2可通过提高移植瘤组织的Caspase-3表达活性来诱导细胞凋亡,抑制肿瘤生长,且效果在一定范围内呈TiO2含量依赖性.光催化纳米TiO2通过诱导肿瘤细胞凋亡和介导炎性反应是其发挥抑癌作用的可能机制.
目的 研究光催化納米二氧化鈦(TiO2)對人鼻嚥癌裸鼠移植瘤模型的治療效果,初步探討其抑癌機製.方法 建立荷人鼻嚥癌裸鼠移植瘤模型,隨機分成6組,包括實驗組:3箇梯度含量的納米TiO2+紫外光照射組；陰性對照組:單純納米TiO2組與單純紫外光照射組；空白對照組.其中納米TiO2懸液採用瘤內註射,經波長範圍330 ～400nm的紫外光處理後24h,取各組瘤組織HE染色作病理分析；透射電鏡觀察腫瘤細胞超微結構的變化及凋亡情況；採用免疫組化檢測腫瘤組織Caspase-3的錶達,採用末耑脫氧覈苷痠轉移酶介導的dUTP缺口末耑標記測定法(TUNEL)檢測細胞凋亡情況,併分析兩者的相關性.結果 病理學分析,納米TiO2光催化治療後移植瘤組織的跼部炎性反應明顯(Ⅱ、Ⅲ級),伴隨炎性反應的是跼部壞死竈形成；而陰性及空白對照組無明顯炎性反應(Ⅰ級).透射電鏡下觀察,實驗組可見納米TiO2顆粒廣汎進入胞質胞覈,腫瘤細胞多有凋亡的形態學改變.實驗組Caspase-3的暘性錶達及TUNEL標記的暘性細胞數顯著多于對照組(P＜0.01),且暘性錶達隨TiO2含量遞增而增彊(P＜0.01).結論 光催化納米TiO2可通過提高移植瘤組織的Caspase-3錶達活性來誘導細胞凋亡,抑製腫瘤生長,且效果在一定範圍內呈TiO2含量依賴性.光催化納米TiO2通過誘導腫瘤細胞凋亡和介導炎性反應是其髮揮抑癌作用的可能機製.
목적 연구광최화납미이양화태(TiO2)대인비인암라서이식류모형적치료효과,초보탐토기억암궤제.방법 건립하인비인암라서이식류모형,수궤분성6조,포괄실험조:3개제도함량적납미TiO2+자외광조사조；음성대조조:단순납미TiO2조여단순자외광조사조；공백대조조.기중납미TiO2현액채용류내주사,경파장범위330 ～400nm적자외광처리후24h,취각조류조직HE염색작병리분석；투사전경관찰종류세포초미결구적변화급조망정황；채용면역조화검측종류조직Caspase-3적표체,채용말단탈양핵감산전이매개도적dUTP결구말단표기측정법(TUNEL)검측세포조망정황,병분석량자적상관성.결과 병이학분석,납미TiO2광최화치료후이식류조직적국부염성반응명현(Ⅱ、Ⅲ급),반수염성반응적시국부배사조형성；이음성급공백대조조무명현염성반응(Ⅰ급).투사전경하관찰,실험조가견납미TiO2과립엄범진입포질포핵,종류세포다유조망적형태학개변.실험조Caspase-3적양성표체급TUNEL표기적양성세포수현저다우대조조(P＜0.01),차양성표체수TiO2함량체증이증강(P＜0.01).결론 광최화납미TiO2가통과제고이식류조직적Caspase-3표체활성래유도세포조망,억제종류생장,차효과재일정범위내정TiO2함량의뢰성.광최화납미TiO2통과유도종류세포조망화개도염성반응시기발휘억암작용적가능궤제.
Objective To study the therapeutic effect of photocatalytic nano-TiO2 on nasopharyngeal carcinoma xenograft in nude mice and underlying mechanism. Methods Nude mice bearing human nasopharyngeal carcinoma xenograft were randomly divided into six groups:nano-TiO2 + UV irradiation( with gradient concentration of nano-TiO2 ) ; nano-TiO2 alone and UV irradiation alone and blank control.The nano-TiO2 suspension was injected into xenografts,and 24 h after UV light with the wave length of 330 -400 nm,all the xenografts were removed and sectioned for HE staining.Ultrastructure and apoptosis of tumor cells in the xenografts were observed by transmission electron microscope (TEM).The expression of Caspase-3 was examined immunohistochemical staining and the apoptosis was detected with TUNEL.Results Pathological analysis showed significant inflammatory responses ( grade Ⅱ and Ⅲ ) with local necrosis occurred in tumor tissues after nano-TiO2 photodynamic therapy,but not in the negative control and blank control.TEM showed the nano-TiO2 particles entered into the cytoplasm and the nucleus of tumor cells and many tumor cells had morphological changes for apoptosis.Significant positive expression of Caspase-3 and TUNEL-positive cells were found in the the xenograts with the treatments of nano-TiO2 + UV irradiation compared to control( P ＜0.01 ),which were enhanced with the increases in nano-TiO2 concentration (P ＜ 0.01 ).Conclusion Photocatalytic nano-TiO2 can inhibit the growth of nasopharyngeal carcinoma xenograft in nude mice by inducing Caspase-3 expression and apoptosis in the tumor cells.
