国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2010年
11期
732-735,封3
,共5页
武钧%毛恩强%李建芳%瞿颖%陈雪华%张明钧%汤耀卿%张圣道
武鈞%毛恩彊%李建芳%瞿穎%陳雪華%張明鈞%湯耀卿%張聖道
무균%모은강%리건방%구영%진설화%장명균%탕요경%장골도
急性胰腺炎%内皮祖细胞%内皮损伤
急性胰腺炎%內皮祖細胞%內皮損傷
급성이선염%내피조세포%내피손상
Acute pancreatitis%Endothelial progenitor cells%Endothelium injury
目的 探讨急性胰腺炎时骨髓及循环内皮祖细胞(EPCs)的数量变化及机制.方法 将BALB/c小鼠随机分为对照组和腹腔注射雨蛙素组,腹腔注射后12 h、24 h、48 h,用流式细胞仪测定骨髓和循环中EPCs数量,分别采用ELISA法和Western blot检测循环中VEGF、TNF-α、ET-1含量及胰腺VEGF表达,TUNEL法原位检测胰腺血管内皮细胞凋亡.结果 腹腔内雨蛙素注射后骨髓和循环中EPCs的数量显著增加(P<0.05)并伴有血浆高水平的VEGF、TNF-α、ET-1(P<0.05),骨髓和循环中EPCs数量与血浆VEGF水平呈线性相关(r分别为0.77和0.67);雨蛙素注射后12 h胰腺VEGF表达明显增加;雨蛙素注射后24 h胰腺血管内皮细胞凋亡明显增加.结论 急性胰腺炎可导致胰腺局部血管内皮损伤,并可动员骨髓内EPCs进入循环.
目的 探討急性胰腺炎時骨髓及循環內皮祖細胞(EPCs)的數量變化及機製.方法 將BALB/c小鼠隨機分為對照組和腹腔註射雨蛙素組,腹腔註射後12 h、24 h、48 h,用流式細胞儀測定骨髓和循環中EPCs數量,分彆採用ELISA法和Western blot檢測循環中VEGF、TNF-α、ET-1含量及胰腺VEGF錶達,TUNEL法原位檢測胰腺血管內皮細胞凋亡.結果 腹腔內雨蛙素註射後骨髓和循環中EPCs的數量顯著增加(P<0.05)併伴有血漿高水平的VEGF、TNF-α、ET-1(P<0.05),骨髓和循環中EPCs數量與血漿VEGF水平呈線性相關(r分彆為0.77和0.67);雨蛙素註射後12 h胰腺VEGF錶達明顯增加;雨蛙素註射後24 h胰腺血管內皮細胞凋亡明顯增加.結論 急性胰腺炎可導緻胰腺跼部血管內皮損傷,併可動員骨髓內EPCs進入循環.
목적 탐토급성이선염시골수급순배내피조세포(EPCs)적수량변화급궤제.방법 장BALB/c소서수궤분위대조조화복강주사우와소조,복강주사후12 h、24 h、48 h,용류식세포의측정골수화순배중EPCs수량,분별채용ELISA법화Western blot검측순배중VEGF、TNF-α、ET-1함량급이선VEGF표체,TUNEL법원위검측이선혈관내피세포조망.결과 복강내우와소주사후골수화순배중EPCs적수량현저증가(P<0.05)병반유혈장고수평적VEGF、TNF-α、ET-1(P<0.05),골수화순배중EPCs수량여혈장VEGF수평정선성상관(r분별위0.77화0.67);우와소주사후12 h이선VEGF표체명현증가;우와소주사후24 h이선혈관내피세포조망명현증가.결론 급성이선염가도치이선국부혈관내피손상,병가동원골수내EPCs진입순배.
Objective To investigate changes in number of endothelial progenitor cells(EPCs)from bone marrow and circulation in mice with acute pancreatitis.Methods BALB/c mice were assigned randomly to saline group and cerulein group.Animals were sacrificed at 12, 24 and 48 hours after injection.Bone marrow and circulating EPCs were detected by flow cyzometric analysis.Plasma VEGF, TNF-α and ET-1 were determined by enzyme-linked immunosorbent assay.The expression of VEGF in the pancreas was assessed by Western blotting.Apoptosis in situ was detected by TUNEL.Results The amounts of EPCs in bone marrow and circulation increased remarkably after cerulein injection(P < 0.05), also the levels of plasma VEGF TNF-α and ET-1(P < 0.05), the EPCs levels in bone marrow and circulation seen in the study closely mirrors the levels of VEGF detected in the circulation(r = 0.77, 0.67 individually).VEGF expression in pancreas was up-regulated after 12 h of cerulein injection compared with that of control group.Apoptosis of endothelial cells also increased in the cerulein group.Conclusion EPCs were mobilized by acute pancreatitis, which may be due to the mobilizing effect of increased levels of VEGF, EPCs may participate in the repair process of injured endothelium induced by acute pancreatitis.
