CAJ | 학술논문

目的探讨AnnexinA5基因多态性(-1C/T)在煤工尘肺患者发病遗传易感性中的作用.方法采用病例-对照研究方法,选择470例汉族煤工尘肺病例为观察对象,428例汉族煤尘接触者为对照,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测AnnexinA5-1C/T位点的基因多态性,分析多态位点与煤工尘肺的关系.结果煤工尘肺组与对照组各基因型分布频率差异无统计学意义(P＞0.05).调整年龄及吸烟混杂因素后,以CC基因型为参比,粉尘暴露时间较长(≥27年)且携带CT/TT基因型者患煤工尘肺的危险性降低(P=0.039),OR值为0.65 (95％CI:0.44～0.98)；对Ⅱ期煤工尘肺病例,调整了年龄、接尘工龄和吸烟率后,携带CT/TT等位基因的个体患煤工尘肺的危险性降低(P±0.028),OR值为0.55 (95％CI:0.34～0.90).结论 AnnexinA5-1C/T多态性位点可能影响中国汉族人群煤工尘肺的发生.
목적 탐토AnnexinA5기인다태성(-1C/T)재매공진폐환자발병유전역감성중적작용.방법 채용병례-대조연구방법,선택470례한족매공진폐병례위관찰대상,428례한족매진접촉자위대조,응용취합매련반응-한제성편단장도다태성(PCR-RFLP)검측AnnexinA5-1C/T위점적기인다태성,분석다태위점여매공진폐적관계.결과 매공진폐조여대조조각기인형분포빈솔차이무통계학의의(P＞0.05).조정년령급흡연혼잡인소후,이CC기인형위삼비,분진폭로시간교장(≥27년)차휴대CT/TT기인형자환매공진폐적위험성강저(P=0.039),OR치위0.65 (95％CI:0.44～0.98)；대Ⅱ기매공진폐병례,조정료년령、접진공령화흡연솔후,휴대CT/TT등위기인적개체환매공진폐적위험성강저(P±0.028),OR치위0.55 (95％CI:0.34～0.90).결론 AnnexinA5-1C/T다태성위점가능영향중국한족인군매공진폐적발생.
Objective To explore the role of-lC/T single nucleotide polymorphism within Annexin A5 gene in the genetic susceptibility to coal worker's pneumoconiosis (CWP).Methods Four hundred and seventy CWP Han chinese patients and 428 Han chinese controls were enclosed in present case-control study.All subjects were exposed to coal dusts.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the -1C/T SNP in Annexin A5 gene for all subjects.The relationship between the -1C/T SNP in Annexin A5 gene and CWP was analyzed.Results CT/TT genotype in -1C/T SNP was associated with a significantly decreased risk of CWP,as compared with the CC genotype among subgroups exposed to coal dusts for ≥27 years (adjusted OR=0.65,95％CI:0.44-0.98,P=0.039) and patients with CWP at stage Ⅱ (adjusted OR=0.55,95％CI:0.34-0.90,P=0.028 ).Conclusion The results of present study suggest that the Annexin A5 -1C/T polymorphism may be involved in the development of CWP in Han Chinese coal miners.