中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
180-182
,共3页
黄陈%裘正军%江弢%朱麟%曹俊%黄克俭
黃陳%裘正軍%江弢%硃麟%曹俊%黃剋儉
황진%구정군%강도%주린%조준%황극검
胰腺癌%RNA干扰%STAT3%生长
胰腺癌%RNA榦擾%STAT3%生長
이선암%RNA간우%STAT3%생장
Pancreatic carcinoma%RNA interference%STAT3%Growth
目的 探讨RNA干扰(RNAi)抑制信号转导与转录激活因子-3(STAT3)对人胰腺癌细胞株SW1990体外生长能力的影响及其机制.方法 构建STAT3短发卡RNA(shRNA)表达载体,稳定转染SW1990细胞,逆转录-聚合酶链反应(RT-PCR)观察STAT3 mRNA表达.噻唑蓝(MTT)和流式细胞仪分别检测细胞增殖和凋亡.RT-PCR检测Cyclin D1和bcl-xL mRNA表达.结果 RN Ai后SW1990细胞中STAT3mRNA表达下降95%;MTT和流式细胞仪结果显示RNAi抑制STAT3后,SW1990细胞增殖能力下降,早期和晚期细胞凋亡比例均升高,分别为(20.97±1.85)%、(23.16±1.94)%;RT-PCR显示RNAi抑制STAT3后,SW1990细胞中Cyclin D1和bcl-xL的mRNA表达分别降低48%和61%.结论 STAT3 shRNA表达载体能有效抑制STAT3,并通过下调bcl-xL和Cyclin D1表达,抑制胰腺癌细胞体外生长能力.
目的 探討RNA榦擾(RNAi)抑製信號轉導與轉錄激活因子-3(STAT3)對人胰腺癌細胞株SW1990體外生長能力的影響及其機製.方法 構建STAT3短髮卡RNA(shRNA)錶達載體,穩定轉染SW1990細胞,逆轉錄-聚閤酶鏈反應(RT-PCR)觀察STAT3 mRNA錶達.噻唑藍(MTT)和流式細胞儀分彆檢測細胞增殖和凋亡.RT-PCR檢測Cyclin D1和bcl-xL mRNA錶達.結果 RN Ai後SW1990細胞中STAT3mRNA錶達下降95%;MTT和流式細胞儀結果顯示RNAi抑製STAT3後,SW1990細胞增殖能力下降,早期和晚期細胞凋亡比例均升高,分彆為(20.97±1.85)%、(23.16±1.94)%;RT-PCR顯示RNAi抑製STAT3後,SW1990細胞中Cyclin D1和bcl-xL的mRNA錶達分彆降低48%和61%.結論 STAT3 shRNA錶達載體能有效抑製STAT3,併通過下調bcl-xL和Cyclin D1錶達,抑製胰腺癌細胞體外生長能力.
목적 탐토RNA간우(RNAi)억제신호전도여전록격활인자-3(STAT3)대인이선암세포주SW1990체외생장능력적영향급기궤제.방법 구건STAT3단발잡RNA(shRNA)표체재체,은정전염SW1990세포,역전록-취합매련반응(RT-PCR)관찰STAT3 mRNA표체.새서람(MTT)화류식세포의분별검측세포증식화조망.RT-PCR검측Cyclin D1화bcl-xL mRNA표체.결과 RN Ai후SW1990세포중STAT3mRNA표체하강95%;MTT화류식세포의결과현시RNAi억제STAT3후,SW1990세포증식능력하강,조기화만기세포조망비례균승고,분별위(20.97±1.85)%、(23.16±1.94)%;RT-PCR현시RNAi억제STAT3후,SW1990세포중Cyclin D1화bcl-xL적mRNA표체분별강저48%화61%.결론 STAT3 shRNA표체재체능유효억제STAT3,병통과하조bcl-xL화Cyclin D1표체,억제이선암세포체외생장능력.
Objective To investigate the effect and mechanism of RNA interference ( RNAi)-mediated signal transducer and activators of transcription 3 ( STAT3 ) gene inhibition on growth of human pancreatic cancer cells in vitro. Methods STAT3 short hairpin RNA (shRNA) expression vector was stably transfected into SW1990 cells. STAT3 mRNA was examined by using reverse transcription-polymerase chain reaction (RT-PCR). Methyl thiazol tetrazolium (MTT) assay and flow cytometry were performed to detect the cell proliferation, and apoptosis, respectively. RT-PCR was performed to detect the mRNA expression of Cyclin D1 and bcl-xL. Results The mRNA expression of STAT3 was decreased by 95% after stable transfection of STAT3 shRNA expressing vectors. Inhibition of STAT3 with RNAi significantly inhibited the growth and proliferation of pancreatic cancer cells, and induced apoptosis in pancreatic cancer cells. The percentage of early apoptotic cells and the late apoptotic cells in SW1990-RNAi cells was increased to (20. 97 ± 1.85)% and (23. 16 ± 1.94)%, respectively. Moreover, the relative Cyclin D1 and bcl-xL mRNA expression in SW1990-RNAi cells was reduced by 48% and 61% as compared with that of the parental SW1990 cells, respectively. Conclusion Inhibition of STAT3 with RNAi can significantly inhibit the growth ability of pancreatic cancer cells through down-regulating bcl-xL and Cyclin D1.
