CAJ | 학술논문

目的检测基质细胞衍生因子-1(stromal cell- derived factor-1,SDF-1)、CD34、微血管密度(MVD)在缺氧缺血型狭长窄蒂皮瓣组织的不同时间、不同面积的表达,以及SDF- 1在狭长窄蒂皮瓣组织血管新生中的作用.方法在5头家猪两侧背部各制作蒂部长宽比为4:2,携带皮瓣面积分别为2 cm×2 cm(A组)、3 cm×3 cm(B组)、4 cm×4 cm(C组)、5 cm×5 cm(D组)和6 cm×6 cm(E组)共5组的窄长蒂皮瓣模型.对5组皮瓣进行大体观察、皮瓣成活情况、光镜下检测MVD、免疫组织化学及酶联免疫技术(ELISA),检测皮瓣远端组织在术后0、3、5、7、14 d时,SDF-1和CD34表达.结果 (1)同一组狭长窄蒂皮瓣,随着时间推移,SDF-1、CD34及MVD表达也随之增加,SDF-1在第5天达最高值(A组124.80±4.05,B组137.85±3.03,C组166.53±2.98,D组72.80±2.63,E组62.79±2.20);CD34在第7天达最高值(A组16.76±0.62,B组17.60±0.72,C组18.48±0.55,D组12.70±0.60,E组11.51±0.70);MVD在第7天达最高值(A组52.45±2.78,B组59.34±3.12,C组61.14±3.35,D组25 25±3.78,E组24.46±7.46),随后逐渐下降.(2)不同组狭长窄蒂皮瓣,随着皮瓣面积的增大,SDF-1、CD34及MVD的表达也随之增加,当面积达5 cm×5 cm时,各项指标不再增加,皮瓣远端出现部分坏死.结论 SDF-1对狭长窄蒂皮瓣成活中的新生血管起一定的调节作用.
목적 검측기질세포연생인자-1(stromal cell- derived factor-1,SDF-1)、CD34、미혈관밀도(MVD)재결양결혈형협장착체피판조직적불동시간、불동면적적표체,이급SDF- 1재협장착체피판조직혈관신생중적작용.방법 재5두가저량측배부각제작체부장관비위4:2,휴대피판면적분별위2 cm×2 cm(A조)、3 cm×3 cm(B조)、4 cm×4 cm(C조)、5 cm×5 cm(D조)화6 cm×6 cm(E조)공5조적착장체피판모형.대5조피판진행대체관찰、피판성활정황、광경하검측MVD、면역조직화학급매련면역기술(ELISA),검측피판원단조직재술후0、3、5、7、14 d시,SDF-1화CD34표체.결과 (1)동일조협장착체피판,수착시간추이,SDF-1、CD34급MVD표체야수지증가,SDF-1재제5천체최고치(A조124.80±4.05,B조137.85±3.03,C조166.53±2.98,D조72.80±2.63,E조62.79±2.20);CD34재제7천체최고치(A조16.76±0.62,B조17.60±0.72,C조18.48±0.55,D조12.70±0.60,E조11.51±0.70);MVD재제7천체최고치(A조52.45±2.78,B조59.34±3.12,C조61.14±3.35,D조25 25±3.78,E조24.46±7.46),수후축점하강.(2)불동조협장착체피판,수착피판면적적증대,SDF-1、CD34급MVD적표체야수지증가,당면적체5 cm×5 cm시,각항지표불재증가,피판원단출현부분배사.결론 SDF-1대협장착체피판성활중적신생혈관기일정적조절작용.
Objective To detect the expression of SDF-1 and CD34,and MVD changes at slen der narrow pedicle flap of hypoxia and ischemia during the survival process,and to investigate the role of SDF-1 at flap neovascularization.Methods The slender narrow pedicle flaps,which pedicle's length-breadth ratio were 4 ∶ 2,and the flap size of each took an area of 2 cm × 2 cm (group A),3 cm ×3 cm (group B),4 cm × 4 cm (group C),5 cm × 5 cm (group D) and 6 cm × 6 cm (group E),were designed and elevated on each pigs' double dorsum.The flaps were served as a hypoxia/ischemia flap model.The survival area,histologic analysis,SDF-1 and CD34 enzyme-linked immunosorbent assay at the distal flap were evaluated at days 0,3,5,7,and 14 after operation,respectively.Results In the each group of flaps,the expression of SDF-1,CD34 and MVD increased with time,reached the plateau level after 5 days (maximal values of SDF-1:124.80 ± 4.05 ingroup A,137.85 ±3.03 in group B,166.53 ± 2.98 in group C,72.80 ± 2.63 in group D and 62.79 ± 2.20 in group E),7 days ( maximal values of CD34:16.76 ± 0.62,17.60 ± 0.72,18.48 ± 0.55,12.70 ± 0.60,and 11.51 ± 0.70,each group),and 7 days (maximal values of MVD:52.45 ± 2.78,59.34 ± 3.12,61.14 ± 3.35,25.25 ± 3.78,and 24.46 ± 7.46,each group),and then gradually decreased.In the different groups of flaps,when the flap area increased,the expression of SDF-1,CD34 and MVD increased,but the parameters decreased at the area of 5 cm × 5 cm,and the flaps were partial necrosis.Conclusions SDF-1 may play an adjusted role in the survival process of the slender narrow pedicle flap.