CAJ | 학술논문

经过单因素试验和响应面综合试验,确定了枯草芽孢杆菌(Bacillus subtilis)ls-45发酵生产玉米多肽的最佳发酵条件:接种量12%,40目玉米蛋白粉,底物浓度5%,初始pH 8.0,装液量100 mL/500 mL三角瓶,培养温度41℃,摇床转速180 r/min,发酵时间63 h.在此条件下玉米肽得率达到最大为82.7%.并对制得的玉米肽进行了初步的分离纯化,经截留分子质量10~4u的中空纤维柱超滤,得到分子质量在10~4u左右的超滤液,其回收率为86.3%,超滤液经SephadexG-25柱分离纯化和相对分子质量分布的测定,共洗脱出4个峰,其玉米肽各级分的相对分子质量分别大致为5128、3715和1513.
경과단인소시험화향응면종합시험,학정료고초아포간균(Bacillus subtilis)ls-45발효생산옥미다태적최가발효조건:접충량12%,40목옥미단백분,저물농도5%,초시pH 8.0,장액량100 mL/500 mL삼각병,배양온도41℃,요상전속180 r/min,발효시간63 h.재차조건하옥미태득솔체도최대위82.7%.병대제득적옥미태진행료초보적분리순화,경절류분자질량10~4u적중공섬유주초려,득도분자질량재10~4u좌우적초려액,기회수솔위86.3%,초려액경SephadexG-25주분리순화화상대분자질량분포적측정,공세탈출4개봉,기옥미태각급분적상대분자질량분별대치위5128、3715화1513.
The conditions for producing corn peptide by fermentation with Bacillus subtilis ls-45 were optimized by adopting the response surface method based on single factor tests.Results show the optimal conditions are inoculum size of Bacillus subtilis ls-45 12%,corn crushed in 40 meshes,substrate concentration 5%,pH 8.0,liquid volume 100 mL/500 mL conical beaker,fermentation temperature 41℃,rotation speed 180 r/min,and fermentation time 63 h.The corn peptide yield rate reaches 82.7%under the conditions.The gained corn peptide was purified by using hollow fiber ultrafiltration column and a ultrafltrate with molecular weight of 104 u was producedthe recovery rate is 86.3%.The uhrafiltrate Was further purified by SephadexG-25 column and the distribution of molecular weight Was analyzed,and four peaks emerged.Results show the relative molecular weights of the corn peptides in all levels are 5128,3715 and 1513.