CAJ | 학술논문

初步研究了摇瓶条件下地衣芽孢杆菌发酵生产3-羟基-2-丁酮的工艺条件.分析了摇瓶发酵中菌体生长、产物合成和pH的变化情况;考察了发酵温度、装液量、种龄、接种量等条件对发酵的影响;研究了地衣芽孢杆菌利用不同碳源发酵生产3-羟基-2-丁酮的情况,选取了最适碳源,在此基础上进行了补糖试验;最后采用正交试验方法对培养基进行了优化.在优化的发酵条件下,采用分批补糖的工艺,摇瓶发酵产量可稳定在20 g/L以上,为3-羟基-2-丁酮的工业化生产提供了一定的基础.
초보연구료요병조건하지의아포간균발효생산3-간기-2-정동적공예조건.분석료요병발효중균체생장、산물합성화pH적변화정황;고찰료발효온도、장액량、충령、접충량등조건대발효적영향;연구료지의아포간균이용불동탄원발효생산3-간기-2-정동적정황,선취료최괄탄원,재차기출상진행료보당시험;최후채용정교시험방법대배양기진행료우화.재우화적발효조건하,채용분비보당적공예,요병발효산량가은정재20 g/L이상,위3-간기-2-정동적공업화생산제공료일정적기출.
The nutritional and environmental conditions for acetoin production from Bacillus licheniformis in flasks were studied in this manuscript. Firstly, the environmental conditions, such as pH value, culture temperature, seed age, volume and inoculation ratio, and nutritional conditions, like carbon and nitrogen sources were carefully investigated. Based on the results of the single factor experiment, the orthogonal experiment was carried to get the optimum medium composition. Then fed-batch fermentation was performed with glucose feeding. The yield of acetoin achieved at 20 g/L in fed-batch fermentation with glucose feeding.