中国运动医学杂志
中國運動醫學雜誌
중국운동의학잡지
CHINESE JOURNAL OF SPORTS MEDICINE
2010年
3期
295-298
,共4页
CKMB%cTnI%缺氧%心肌细胞
CKMB%cTnI%缺氧%心肌細胞
CKMB%cTnI%결양%심기세포
CKMB%cTnI%hypoxia%myocardial cells
目的:研究in vitro 心肌细胞在长时间缺氧刺激后,CKMB和cTnI的表达特点,为探讨缺氧诱发心脏高表达CKMB和cTnI的机制提供参考.方法:采用体外培养新生Wister大鼠心肌细胞模型,两步法RT-PCR和Western Blotting分别检测上清和胞内HIF-1α和CKMB、cTnI在缺氧培养40分钟后连续时间点的表达变化.结果:缺氧后15min,上清CKMB表达显著升高,后随时间推移缓慢升高;胞内表达在0min～24h均显著高于上清(P<0.05),1h后达峰值.上清cTnI浓度在缺氧15min后急剧升高,24h后达峰值;胞内cTnI浓度在缺氧45min后达峰值;缺氧心肌细胞内、上清cTnI表达在15min～48h均有显著性变化(P<0.05),且呈近似同步性.胞内cTnl浓度在24h后低于上清.结论:单纯长时间缺氧刺激可引起in vitro心肌细胞内高表达CKMB和cTnI,并向胞外释放,推测缺氧导致胞膜渗漏是主要释放机制.
目的:研究in vitro 心肌細胞在長時間缺氧刺激後,CKMB和cTnI的錶達特點,為探討缺氧誘髮心髒高錶達CKMB和cTnI的機製提供參攷.方法:採用體外培養新生Wister大鼠心肌細胞模型,兩步法RT-PCR和Western Blotting分彆檢測上清和胞內HIF-1α和CKMB、cTnI在缺氧培養40分鐘後連續時間點的錶達變化.結果:缺氧後15min,上清CKMB錶達顯著升高,後隨時間推移緩慢升高;胞內錶達在0min～24h均顯著高于上清(P<0.05),1h後達峰值.上清cTnI濃度在缺氧15min後急劇升高,24h後達峰值;胞內cTnI濃度在缺氧45min後達峰值;缺氧心肌細胞內、上清cTnI錶達在15min～48h均有顯著性變化(P<0.05),且呈近似同步性.胞內cTnl濃度在24h後低于上清.結論:單純長時間缺氧刺激可引起in vitro心肌細胞內高錶達CKMB和cTnI,併嚮胞外釋放,推測缺氧導緻胞膜滲漏是主要釋放機製.
목적:연구in vitro 심기세포재장시간결양자격후,CKMB화cTnI적표체특점,위탐토결양유발심장고표체CKMB화cTnI적궤제제공삼고.방법:채용체외배양신생Wister대서심기세포모형,량보법RT-PCR화Western Blotting분별검측상청화포내HIF-1α화CKMB、cTnI재결양배양40분종후련속시간점적표체변화.결과:결양후15min,상청CKMB표체현저승고,후수시간추이완만승고;포내표체재0min～24h균현저고우상청(P<0.05),1h후체봉치.상청cTnI농도재결양15min후급극승고,24h후체봉치;포내cTnI농도재결양45min후체봉치;결양심기세포내、상청cTnI표체재15min～48h균유현저성변화(P<0.05),차정근사동보성.포내cTnl농도재24h후저우상청.결론:단순장시간결양자격가인기in vitro심기세포내고표체CKMB화cTnI,병향포외석방,추측결양도치포막삼루시주요석방궤제.
Objective To study the expression character of CKMB and cTnI presented in long-time in vitro hypoxic myocardial cells,and provide theoretical consult in understanding the mechanism of highly expressing CKMB and cTnI induced by hypoxia in hearts.Methods The myocardial cells were sampled from newborn Wister mice.Two-step RT-PCR and Western Blotting were applied to detect the consecutive expression variation of HIF-1α and CKMB.cTnI both in the supernatant and inner of incubating cells after 40-minute hypoxia intervention.Results There had CKMB appeared in the supernatant within 15min after hypoxic stimulation.with slow increasing along the time.The intracellular CKMB expression got its peak point within 1h,and then descended.1k intracellular CKMB concentration was significantly higher than that of the supernatant of hypoxic myocardial cells within Omin-24h(P<0.05).The cTnI concentration of supematant steeply increased after 1 5min,reaching the peak point within 24h.The intracellular cTnI concentration reached the peaK point within 45min.The significant differences always existed for the cTnI expression between intra-and extra-myocardial cells within 15min-48h(P<0.05),and their increasing tendencies nearly showed synchronism.The intracellular cTnI concentration was lower than that of supernatant 24h later.Conclusion The single persistent hypoxic stimulation could induce the intensive intracellular expression and delivery of CKMB and cTnI in vitro myocardial cells.and the latter probably was caused by the leakage of cell membrane originally provoked by hypoxia.
