棘球幼病,肝%寄生虫感染%免疫逃避%Th17/Treg失衡
棘毬幼病,肝%寄生蟲感染%免疫逃避%Th17/Treg失衡
극구유병,간%기생충감염%면역도피%Th17/Treg실형
Echinococcosis,hepatic%Parasitic infection%Immune evasion%Th17/Treg imbalance
目的 探讨辅助性T细胞17(Th17)和调节性T细胞(Treg细胞)在肝包虫病免疫逃避中的作用.方法 前瞻性分析2008年8月至2009年9月新疆医科大学第一附属医院74例受试者的临床资料,将74例受试者分为4组:健康对照组20例,肝囊型包虫病组21例,肝复发囊型包虫病组15例和肝泡型包虫病组18例.应用ELISA法检测各组受试者血清中Treg相关细胞因子转化生长因子(TGF-β1)和IL-10,Th17细胞相关细胞因子IL-17和IL-23的表达,并用单因素方差分析,两两比较采用LSD-t法和Pearson相关性检验分析其结果.结果 IL-17在健康对照组、肝囊型包虫病组、肝复发囊型包虫病组和肝泡型包虫病组的表达分别为(16±5)、(13±4)、(13±5)和(11±3)ng/L,4组比较,差异有统计学意义(F=6.35,P<0.05);而肝囊型包虫病组与肝复发囊型包虫病组比较,差异无统计学意义(t=0.22,P＞0.05).IL-23在健康对照组、肝囊型包虫病组、肝复发囊型包虫病组和肝泡型包虫病组的表达分别为(139±50)、(106±53)、(107±48)和(72±27)ng/L,4组比较,差异有统计学意义(F=6.74,P<0.05);而肝囊型包虫病组与肝复发囊型包虫病组比较,差异无统计学意义(t=0.02,P＞0.05).IL-10在健康对照组、肝囊型包虫病组、肝复发囊型包虫病组和肝泡型包虫病组的表达分别为(3.1±0.8)、(4.3±2.0)、(4.2±1.4)和(5.5±2.2)ng/L,4组比较,差异有统计学意义(F=9.78,P<0.05);而肝囊型包虫病组与肝复发囊型包虫病组比较,差异无统计学意义(t=0.14,P＞0.05);TGF-β1在健康对照组、肝囊型包虫病组、肝复发囊型包虫病组和肝泡型包虫病组的表达分别为(26±7)、(37±7)、(33±9)和(38±7)μg/L,4组比较,差异有统计学意义(F=6.73,P<0.05);而3种肝包虫病组比较,差异无统计学意义(t=0.56,1.81,P＞0.05).Th17/Treg(IL-17/IL-10)在健康对照组、肝囊型包虫病组、肝复发囊型包虫病组和肝泡型包虫病组的表达比例分别为5.7±2.6、3.6±1.5、3.4±1.9和2.1±0.7,4组比较,差异有统计学意义(F=13.76,P<0.05);而肝囊型包虫病组与肝复发囊型包虫病组比较,差异无统计学意义(t=0.23,P＞0.05).血清中,IL-17和TGF-β1呈负相关(r=-0.23,P<0.05);IL-17和IL-23呈正相关(r=0.70,P<0.05);IL-10和TGF-β1呈正相关(r=0.46,P<0.05).结论 Th17/Treg相关细胞因子平衡在肝泡型和肝囊型包虫病患者中明显向Treg应答偏倚,Th17/Treg相关细胞因子失衡可能参与肝包虫所致免疫逃避.
目的 探討輔助性T細胞17(Th17)和調節性T細胞(Treg細胞)在肝包蟲病免疫逃避中的作用.方法 前瞻性分析2008年8月至2009年9月新疆醫科大學第一附屬醫院74例受試者的臨床資料,將74例受試者分為4組:健康對照組20例,肝囊型包蟲病組21例,肝複髮囊型包蟲病組15例和肝泡型包蟲病組18例.應用ELISA法檢測各組受試者血清中Treg相關細胞因子轉化生長因子(TGF-β1)和IL-10,Th17細胞相關細胞因子IL-17和IL-23的錶達,併用單因素方差分析,兩兩比較採用LSD-t法和Pearson相關性檢驗分析其結果.結果 IL-17在健康對照組、肝囊型包蟲病組、肝複髮囊型包蟲病組和肝泡型包蟲病組的錶達分彆為(16±5)、(13±4)、(13±5)和(11±3)ng/L,4組比較,差異有統計學意義(F=6.35,P<0.05);而肝囊型包蟲病組與肝複髮囊型包蟲病組比較,差異無統計學意義(t=0.22,P＞0.05).IL-23在健康對照組、肝囊型包蟲病組、肝複髮囊型包蟲病組和肝泡型包蟲病組的錶達分彆為(139±50)、(106±53)、(107±48)和(72±27)ng/L,4組比較,差異有統計學意義(F=6.74,P<0.05);而肝囊型包蟲病組與肝複髮囊型包蟲病組比較,差異無統計學意義(t=0.02,P＞0.05).IL-10在健康對照組、肝囊型包蟲病組、肝複髮囊型包蟲病組和肝泡型包蟲病組的錶達分彆為(3.1±0.8)、(4.3±2.0)、(4.2±1.4)和(5.5±2.2)ng/L,4組比較,差異有統計學意義(F=9.78,P<0.05);而肝囊型包蟲病組與肝複髮囊型包蟲病組比較,差異無統計學意義(t=0.14,P＞0.05);TGF-β1在健康對照組、肝囊型包蟲病組、肝複髮囊型包蟲病組和肝泡型包蟲病組的錶達分彆為(26±7)、(37±7)、(33±9)和(38±7)μg/L,4組比較,差異有統計學意義(F=6.73,P<0.05);而3種肝包蟲病組比較,差異無統計學意義(t=0.56,1.81,P＞0.05).Th17/Treg(IL-17/IL-10)在健康對照組、肝囊型包蟲病組、肝複髮囊型包蟲病組和肝泡型包蟲病組的錶達比例分彆為5.7±2.6、3.6±1.5、3.4±1.9和2.1±0.7,4組比較,差異有統計學意義(F=13.76,P<0.05);而肝囊型包蟲病組與肝複髮囊型包蟲病組比較,差異無統計學意義(t=0.23,P＞0.05).血清中,IL-17和TGF-β1呈負相關(r=-0.23,P<0.05);IL-17和IL-23呈正相關(r=0.70,P<0.05);IL-10和TGF-β1呈正相關(r=0.46,P<0.05).結論 Th17/Treg相關細胞因子平衡在肝泡型和肝囊型包蟲病患者中明顯嚮Treg應答偏倚,Th17/Treg相關細胞因子失衡可能參與肝包蟲所緻免疫逃避.
목적 탐토보조성T세포17(Th17)화조절성T세포(Treg세포)재간포충병면역도피중적작용.방법 전첨성분석2008년8월지2009년9월신강의과대학제일부속의원74례수시자적림상자료,장74례수시자분위4조:건강대조조20례,간낭형포충병조21례,간복발낭형포충병조15례화간포형포충병조18례.응용ELISA법검측각조수시자혈청중Treg상관세포인자전화생장인자(TGF-β1)화IL-10,Th17세포상관세포인자IL-17화IL-23적표체,병용단인소방차분석,량량비교채용LSD-t법화Pearson상관성검험분석기결과.결과 IL-17재건강대조조、간낭형포충병조、간복발낭형포충병조화간포형포충병조적표체분별위(16±5)、(13±4)、(13±5)화(11±3)ng/L,4조비교,차이유통계학의의(F=6.35,P<0.05);이간낭형포충병조여간복발낭형포충병조비교,차이무통계학의의(t=0.22,P＞0.05).IL-23재건강대조조、간낭형포충병조、간복발낭형포충병조화간포형포충병조적표체분별위(139±50)、(106±53)、(107±48)화(72±27)ng/L,4조비교,차이유통계학의의(F=6.74,P<0.05);이간낭형포충병조여간복발낭형포충병조비교,차이무통계학의의(t=0.02,P＞0.05).IL-10재건강대조조、간낭형포충병조、간복발낭형포충병조화간포형포충병조적표체분별위(3.1±0.8)、(4.3±2.0)、(4.2±1.4)화(5.5±2.2)ng/L,4조비교,차이유통계학의의(F=9.78,P<0.05);이간낭형포충병조여간복발낭형포충병조비교,차이무통계학의의(t=0.14,P＞0.05);TGF-β1재건강대조조、간낭형포충병조、간복발낭형포충병조화간포형포충병조적표체분별위(26±7)、(37±7)、(33±9)화(38±7)μg/L,4조비교,차이유통계학의의(F=6.73,P<0.05);이3충간포충병조비교,차이무통계학의의(t=0.56,1.81,P＞0.05).Th17/Treg(IL-17/IL-10)재건강대조조、간낭형포충병조、간복발낭형포충병조화간포형포충병조적표체비례분별위5.7±2.6、3.6±1.5、3.4±1.9화2.1±0.7,4조비교,차이유통계학의의(F=13.76,P<0.05);이간낭형포충병조여간복발낭형포충병조비교,차이무통계학의의(t=0.23,P＞0.05).혈청중,IL-17화TGF-β1정부상관(r=-0.23,P<0.05);IL-17화IL-23정정상관(r=0.70,P<0.05);IL-10화TGF-β1정정상관(r=0.46,P<0.05).결론 Th17/Treg상관세포인자평형재간포형화간낭형포충병환자중명현향Treg응답편의,Th17/Treg상관세포인자실형가능삼여간포충소치면역도피.
Objective To investigate the effects of Th17 cells and Treg cells on immune evasion in patients with hepatic hydatid disease. Methods From August 2008 to September 2009, 54 patients with hepatic hydatid disease who were treated at the First Affiliated Hospital of Xinjiang Medical University and 20 healthy people (control group) were enrolled in this study. Of the 54 patients, 21 had liver cystic enchinococcosis (CE)(CE group), 15 had recurrent cystic echinococcosis (RCE) (RCE group) and 18 had liver alveolar echinococcosis(AE) (AE group). The serum concentrations of interleukin-17 (IL-17), IL-23, transforming growth factor-β1(TGF-β1) and IL-10 were measured by enzyme-linked immunosorbent assay. All data were analysed by one-way analysis of variance, LSD-t test and Pearson correlation analysis. Results Serum IL-17 levels were significantlylower in the AE group [(11±3)ng/L], CE group [(13±4) ng/L] and RCE group [(13 ±5) ng/L]compared with those in the control group [(16±5) ng/L] ( F = 6.53, P ＜ 0.05 ). There was no significant difference in serum IL-17 levels between the CE and RCE groups (t =0.22, P ＞0.05). Serum levels of IL-23were also lower in the AE group [(72±27) ng/L], CE group [( 106±53) ng/L] and RCE group [( 107±48 ) ng/L] compared with those in the control group [( 139±50) ngg/L] ( F = 6.74, P ＜ 0.05 ), while there was no significant difference between the CE and RCE groups (t =0.02, P＞0.05). The serum levels of IL-10 were significantly higher in the AE group [(5.5±2.2) ng/L], CE group [(4.3±2.0) ng/L] and RCE group [(4.2 ± 1.4) ng/L] compared with those in the control group [(3.1 ± 0.8 ) ng/L] ( F = 9.78, P ＜ 0.05 ),with no significant differences between the CE and RCE groups ( t = 0.14, P ＞ 0.05 ). TGF-β1 levels were significantly higher in the AE group [(38±7) μg/L], CE group [(37±7) μg/L] and RCE group [(33±9) μg/L]compared with those in the control group [( 26±7) μg,/L] ( F = 6.73, P＜ 0.05 ), with no significant difference among the AE, CE and RCE groups ( t = 0.56, 1.81, P ＞ 0.05 ). The Th17/Treg (IL-17/IL-10) ratio was significantly decreased in the AE group ( 2.1 ± 0.7 ), CE group ( 3.6 ± 1.5 ) and RCE group ( 3.4 ± 1.9)compared with that in the control group (5.7 ± 2.6) ( F = 13.76, P ＜ 0.05 ), while no significant difference was found between the CE and RCE groups (t = 0.23, P ＞ 0.05). The serum concentrations of IL-17 were negatively correlated with TGF-β1 ( r = - 0.23, P ＜ 0.05 ) and positively correlated with IL-23 ( r = 0.70, P ＜ 0.05 ).Serum concentrations of IL-10 were positively correlated with TGF-β1 ( r = 0.46, P ＜ 0.05 ). Conclusion The overwhelming expression of Treg related cytokines disrupts the Th17/Treg balance in patients with AE or CE,which may have a potential role in immune evasion in the progress of hydatid disease.
