白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2010年
5期
301-303,312
,共4页
李明利%苏丽萍%张景萍%赵瑾%解菊芬%李茜汝%郑美婧%周永安
李明利%囌麗萍%張景萍%趙瑾%解菊芬%李茜汝%鄭美婧%週永安
리명리%소려평%장경평%조근%해국분%리천여%정미청%주영안
白血病,淋巴细胞,慢性%原位杂交,荧光%基因缺失
白血病,淋巴細胞,慢性%原位雜交,熒光%基因缺失
백혈병,림파세포,만성%원위잡교,형광%기인결실
Leukemia,lymphocytic,chronic%In situ hybridization,fluorescence%Gene deletion
目的 探讨慢性淋巴细胞白血病(CLL)患者中ATM基因缺失情况及其与临床分期的相关性.方法 以骨髓涂片档案片为标本,运用荧光原位杂交(FISH)技术和SpectrumOrange~(TM)标记的位于11q22.3的序列特异性DNA探针ATM对28例初诊的CLL患者进行了ATM缺失的检测,临床分期按照Binet分期方法 ,采用Fisher确切概率法分析ATM基因缺失与患者初诊时临床特征间的关系.结果 FISH分析中,28例CLL患者中有4例(14.3%)检出ATM基因缺失,9例Binet A期患者中1例(11.1%)存在del(ATM)异常;8例Binet B期患者中1例(12.5%)存在异常;11例Binet C期患者中2例(18.2%)存在异常.Fisher确切概率法分析显示ATM基因缺失与年龄、性别、临床分期无明显相关性(P>0.05).结论 以骨髓涂片档案片为样本进行FISH检测,方法 简便,结果 可靠,便于血液系统克隆性疾病的回顾性遗传学分析,ATM基因缺失是CLL患者常见的细胞遗传学改变,其对中国CLL患者的预后预测价值有待进一步深入研究.
目的 探討慢性淋巴細胞白血病(CLL)患者中ATM基因缺失情況及其與臨床分期的相關性.方法 以骨髓塗片檔案片為標本,運用熒光原位雜交(FISH)技術和SpectrumOrange~(TM)標記的位于11q22.3的序列特異性DNA探針ATM對28例初診的CLL患者進行瞭ATM缺失的檢測,臨床分期按照Binet分期方法 ,採用Fisher確切概率法分析ATM基因缺失與患者初診時臨床特徵間的關繫.結果 FISH分析中,28例CLL患者中有4例(14.3%)檢齣ATM基因缺失,9例Binet A期患者中1例(11.1%)存在del(ATM)異常;8例Binet B期患者中1例(12.5%)存在異常;11例Binet C期患者中2例(18.2%)存在異常.Fisher確切概率法分析顯示ATM基因缺失與年齡、性彆、臨床分期無明顯相關性(P>0.05).結論 以骨髓塗片檔案片為樣本進行FISH檢測,方法 簡便,結果 可靠,便于血液繫統剋隆性疾病的迴顧性遺傳學分析,ATM基因缺失是CLL患者常見的細胞遺傳學改變,其對中國CLL患者的預後預測價值有待進一步深入研究.
목적 탐토만성림파세포백혈병(CLL)환자중ATM기인결실정황급기여림상분기적상관성.방법 이골수도편당안편위표본,운용형광원위잡교(FISH)기술화SpectrumOrange~(TM)표기적위우11q22.3적서렬특이성DNA탐침ATM대28례초진적CLL환자진행료ATM결실적검측,림상분기안조Binet분기방법 ,채용Fisher학절개솔법분석ATM기인결실여환자초진시림상특정간적관계.결과 FISH분석중,28례CLL환자중유4례(14.3%)검출ATM기인결실,9례Binet A기환자중1례(11.1%)존재del(ATM)이상;8례Binet B기환자중1례(12.5%)존재이상;11례Binet C기환자중2례(18.2%)존재이상.Fisher학절개솔법분석현시ATM기인결실여년령、성별、림상분기무명현상관성(P>0.05).결론 이골수도편당안편위양본진행FISH검측,방법 간편,결과 가고,편우혈액계통극륭성질병적회고성유전학분석,ATM기인결실시CLL환자상견적세포유전학개변,기대중국CLL환자적예후예측개치유대진일보심입연구.
Objective To investigate ATM deletion [del (ATM)] in chronic lymphocytic leukemia (CLL) and study its correlation with the clinical stage. Methods Spectrum Orange~(TM) labeled sequence specific DNA probe for ATM locus on 11q22.3 and fluorescence in Situ hybridization (FISH) was used to examine del (ATM) in 28 newly diagnose patients with CLL. FISH analysis were performed on bone marrow smears. Clinical staging was done according to Binet Method.Fisher exact propability was used to study the relations between del (ATM) and clinical feature. Results 4 out of the 28 cases were found with deletion of ATM. The incidence of del (ATM) in BinetA, BinetB and BinetC was 1/9 (11.1 %), 1/8 (12.5 %), 2/11 (18.2 %), respectively. Fisher exact propability showed that deletion of ATM was not associated with its clinical feature. Conclusion Application of FISH on archival bone marrow smears is a simple, liable method, and can be readly used to retrospective study of clonal blood system diseases. Deletion of ATM was common cytogenetic changes in CLL patients.And the significance of del (ATM) in the prognosis of CLL in China needs to be further investigated.
