白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2011年
7期
410-411,414
,共3页
叶芳%乔振华%郭宏锋%杨林花
葉芳%喬振華%郭宏鋒%楊林花
협방%교진화%곽굉봉%양림화
造血干细胞移植%杀伤细胞,天然%重建%非清髓
造血榦細胞移植%殺傷細胞,天然%重建%非清髓
조혈간세포이식%살상세포,천연%중건%비청수
Hematopoietic stem cell transplantion%Killer cells,natural%Reconstitution%nonmyeloablative
目的 研究非清髓性异基因造血干细胞移植(NAST)后早期自然杀伤(NK)细胞的重建情况.方法 分别用流式细胞术直接免疫荧光法、T细胞活化实验、四甲基偶氮唑蓝比色(MTT)法检测10例NAST后白血病患者及10位健康对照者细胞表面标志及体外免疫功能.结果 移植后28～35 d,患者外周血CD+3、CD+4细胞比例降低,分别为(2.03±15.60)%和(22.69±12.29)%,CD+8细胞比例正常或增高,平均为(29.26±8.99)%;T细胞对有丝分裂原反应减低,其增殖反应刺激指数为94.60±44.87;NK细胞比例在正常范围或增高,为(18.77±9.11)%;NK细胞表面IL-2R表达阳性率增高,平均为(3.71±2.23)%,和正常对照组的(2.05±0.94)%相比差异有统计学意义(t=2.116,P=0.044);部分患者NK细胞活性明显增强,移植组与健康对照组分别为(25.30±12.39)%和(16.60±3.53)%(t=2.135,P=0.047).结论 NK细胞在NAST后早期即恢复,当特异性免疫功能仍低下时,它可能在机体抗感染和抗肿瘤中发挥着重要作用.
目的 研究非清髓性異基因造血榦細胞移植(NAST)後早期自然殺傷(NK)細胞的重建情況.方法 分彆用流式細胞術直接免疫熒光法、T細胞活化實驗、四甲基偶氮唑藍比色(MTT)法檢測10例NAST後白血病患者及10位健康對照者細胞錶麵標誌及體外免疫功能.結果 移植後28～35 d,患者外週血CD+3、CD+4細胞比例降低,分彆為(2.03±15.60)%和(22.69±12.29)%,CD+8細胞比例正常或增高,平均為(29.26±8.99)%;T細胞對有絲分裂原反應減低,其增殖反應刺激指數為94.60±44.87;NK細胞比例在正常範圍或增高,為(18.77±9.11)%;NK細胞錶麵IL-2R錶達暘性率增高,平均為(3.71±2.23)%,和正常對照組的(2.05±0.94)%相比差異有統計學意義(t=2.116,P=0.044);部分患者NK細胞活性明顯增彊,移植組與健康對照組分彆為(25.30±12.39)%和(16.60±3.53)%(t=2.135,P=0.047).結論 NK細胞在NAST後早期即恢複,噹特異性免疫功能仍低下時,它可能在機體抗感染和抗腫瘤中髮揮著重要作用.
목적 연구비청수성이기인조혈간세포이식(NAST)후조기자연살상(NK)세포적중건정황.방법 분별용류식세포술직접면역형광법、T세포활화실험、사갑기우담서람비색(MTT)법검측10례NAST후백혈병환자급10위건강대조자세포표면표지급체외면역공능.결과 이식후28～35 d,환자외주혈CD+3、CD+4세포비례강저,분별위(2.03±15.60)%화(22.69±12.29)%,CD+8세포비례정상혹증고,평균위(29.26±8.99)%;T세포대유사분렬원반응감저,기증식반응자격지수위94.60±44.87;NK세포비례재정상범위혹증고,위(18.77±9.11)%;NK세포표면IL-2R표체양성솔증고,평균위(3.71±2.23)%,화정상대조조적(2.05±0.94)%상비차이유통계학의의(t=2.116,P=0.044);부분환자NK세포활성명현증강,이식조여건강대조조분별위(25.30±12.39)%화(16.60±3.53)%(t=2.135,P=0.047).결론 NK세포재NAST후조기즉회복,당특이성면역공능잉저하시,타가능재궤체항감염화항종류중발휘착중요작용.
Objective To study the reconstitution of nature kill cell early after non-myeloablative allogeneic stem cell transplantation (NAST). Methods Cell phenotypes and in vitro immune functions were analyzed by direct immune fluorescence with FCM, T-cell activation test and MTT assay, respectively. Results The percentages of CD+3, CD+4 cells were low, (2.03±15.60) % and (22.69±12.29)%, respectively, while CD+8 lymphocytes were normal or high [(29.26±8.99)%] 1 month after NAST. Proliferative response to a T lymphocyte activator (PHA) was blunted, 94.60±44.87. The percentage of NK cells was within normal limits or high (n=7) in allotransplanted patients. It is (18.77±9.11) %. The percentage of CD+56 NK cells expressing IL-2R (CD25) was high and values obtained from transplanted patients were significantly different from control values . They are (3.71±2.23) % (t = 2.116, P = 0.044). NK cell activity in part of patients was higher than that observed in control cells (25.30±12.39) % vs (16.60±3.53) % (t = 2.135, P= 0.047). Conclusion NK cell has recovered early after transplantation and may be particularly relevant as a first line of defence in immunosurveillance against neoplastic cells or microbial infections, until a full reconstitution of T cellmediated immune response can be achieved.
