CAJ | 학술논문

目的研究树突细胞特异性细胞间黏附分子-3结合非整合素因子(dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin,DC-SIGN)启动子区-139和-336位点多态性与HIV易感性、感染途径以及疾病进展的关系.方法比较160例HIV感染者和178名健康对照人群DC-SIGN启动子区-139位点和-336位点基因型分布的差异,采用Spearman秩和相关检验分析DC-SIGN启动子区-139和-336多态性与HIV感染的关系.结果在160例HIV感染者中,出现92例(57.5%)-139C和68例(42.5%)-139T,29例(18.1%)-336C和131例(81.9%)-336T.-139T/C和-336T/C基因型分布在HIV感染者与健康对照者中的差异无统计学意义(χ2=0.121和1.754,P值均＞0.05).通过性接触或静脉注射毒品而感染HIV的患者中,-139T/C和-336T/C分布差异无统计学意义(χ2=0.435和0.103,P值均＞0.05).在所有研究对象中,-139C基因型的个体多伴随-336C基因型(r=0.359,P＜0.01).在外周血CD4+T细胞计数≤50个/μL的患者中,-139T基因型出现的频率(27.9%)高于-139C基因型(23.0%)(χ2=4.055,P＜0.05).-139T/C和-336T/C对HIV RNA载量影响不明显(t=-0.643和-1.637,P值均＞0.05).结论 DC-SIGN启动子区-336C常与-139C耦联出现;-139或-336位点多态性与HIV的易感性和感染途径无明显相关性,但-139T基因型患者更易出现CD4+T细胞数量的下降.
목적 연구수돌세포특이성세포간점부분자-3결합비정합소인자(dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin,DC-SIGN)계동자구-139화-336위점다태성여HIV역감성、감염도경이급질병진전적관계.방법 비교160례HIV감염자화178명건강대조인군DC-SIGN계동자구-139위점화-336위점기인형분포적차이,채용Spearman질화상관검험분석DC-SIGN계동자구-139화-336다태성여HIV감염적관계.결과 재160례HIV감염자중,출현92례(57.5%)-139C화68례(42.5%)-139T,29례(18.1%)-336C화131례(81.9%)-336T.-139T/C화-336T/C기인형분포재HIV감염자여건강대조자중적차이무통계학의의(χ2=0.121화1.754,P치균＞0.05).통과성접촉혹정맥주사독품이감염HIV적환자중,-139T/C화-336T/C분포차이무통계학의의(χ2=0.435화0.103,P치균＞0.05).재소유연구대상중,-139C기인형적개체다반수-336C기인형(r=0.359,P＜0.01).재외주혈CD4+T세포계수≤50개/μL적환자중,-139T기인형출현적빈솔(27.9%)고우-139C기인형(23.0%)(χ2=4.055,P＜0.05).-139T/C화-336T/C대HIV RNA재량영향불명현(t=-0.643화-1.637,P치균＞0.05).결론 DC-SIGN계동자구-336C상여-139C우련출현;-139혹-336위점다태성여HIV적역감성화감염도경무명현상관성,단-139T기인형환자경역출현CD4+T세포수량적하강.
Objective To investigate the polymorphisms of-139 and -336 nucleotides in dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) promoter region in context of HIV susceptibility, infection routines and HIV/AIDS progress. Methods Polymorphisms of -139 and -336 nucleotides in DC-SIGN were examined in 160 HIV-positive subjects and 178 healthy controls;the Spearman test was performed to analyze their associations with HIV infection status. Results In 160 HIV-positive subjects, there were 92 (57.5%) with-139C, 68 (42.5%) with-139T, 29 (18.1%) with-336C and 131 (81.9%) with -336T. The frequencies of -139T/C and -336T/C in HIV-positive subjects were similar to those in the healthy controls (χ2 =0. 121 and 1. 754, P ＞0.05 ). No differences were found in the distribution of -139T/C or -336T/C in HIV-positive subjects infected via sex intercourse or intravenous drug (χ2 =0. 435 and 0. 103, P ＞ 0. 05 ). -139C was usually companied with -336C ( r = 0. 359, P ＜ 0.01 ).-139T (27.9%) were more frequently presented in patients with CD4 +T cells ≤50 cells/μL than -139C( 23.0%, χ2 = 4.055, P ＜ 0.05 ). -139T/C and -336T/C were not related to HIV RNA levels ( t = - 0. 643and - 1. 637, P ＞ 0.05). Conclusions Genotype -139C in DC-SIGN promoter region usually coexist with -336C. Polymorphisms of -139 and -336 are not related to HIV susceptibilities or HIV infection routes.-139T genotype may be related to serious depletion on CD4 + T cells.