CAJ | 학술논문

目的研究外源性血管内皮生长因子(VEGF)对大鼠脑出血后血肿周围区新生血管、GAP - 43表达及神经功能恢复的影响,探讨外源性VEGF对脑出血的保护机制.方法采用二步自体血脑内注入法建立脑出血动物模型.36只雄性SD大鼠随机分为假手术组、模型组、治疗组.治疗组于术后8-15 d连续经腹腔给予外源性VEGF,给药后15d和30 d分别采用免疫组化法检测CD34、GAP -43的表达.用CD34的表达来反映血管新生情况,用GAP - 43、Longa神经功能评分反映神经损伤及修复情况.结果治疗组CD34阳性微血管密度及血管场面积在15 d和30 d均高于模型组及假手术组(P＜0.01).治疗组15 d及30 d的GAP - 43阳性细胞表达率及阳性颗粒所占的面积比上调,与模型组、假手术组比较差异有统计学意义(P＜0.01),Longa评分提示治疗组神经功能恢复提前于模型组(P＜0.01).结论外源性VEGF可促进新生血管生长,提高受损中枢神经系统GAP- 43表达,加快神经功能的恢复.
목적 연구외원성혈관내피생장인자(VEGF)대대서뇌출혈후혈종주위구신생혈관、GAP - 43표체급신경공능회복적영향,탐토외원성VEGF대뇌출혈적보호궤제.방법 채용이보자체혈뇌내주입법건립뇌출혈동물모형.36지웅성SD대서수궤분위가수술조、모형조、치료조.치료조우술후8-15 d련속경복강급여외원성VEGF,급약후15d화30 d분별채용면역조화법검측CD34、GAP -43적표체.용CD34적표체래반영혈관신생정황,용GAP - 43、Longa신경공능평분반영신경손상급수복정황.결과 치료조CD34양성미혈관밀도급혈관장면적재15 d화30 d균고우모형조급가수술조(P＜0.01).치료조15 d급30 d적GAP - 43양성세포표체솔급양성과립소점적면적비상조,여모형조、가수술조비교차이유통계학의의(P＜0.01),Longa평분제시치료조신경공능회복제전우모형조(P＜0.01).결론 외원성VEGF가촉진신생혈관생장,제고수손중추신경계통GAP- 43표체,가쾌신경공능적회복.
Objective To study the effects of exogenous VEGF on cerebral angiogenesis,the expression of GAP-43 and nerve functional recovery in rat with intracerebral hemorrhage (ICH),to reveal the mechanism of neuroprotection by exogcnous VEGF.Methods The model of ICH was established in rats by two - step intracerebral injection of autogenous blood.36 male Spranue - Dawley rats were randomly divided into sham- operated group,ICH model group and treatment group.The treatment group was inject with VEGF intraperitoneally during postoperative Day8 to Day15.The expression of CD34,GAP- 43 were detected by immunohistochemistry on Day15 and Day30.Microvascular production was measured by expression changes of CD34.Neurotrosis and neural Repair was measured by expression changes of GAP -34 and Longa evaluation.Results the expression of CD34 + microvascular density and vascular field area of treatment group in Day15 and Day30 are significantly higher than those of model group and sham -operated group ( P ＜ 0.01 ).The rate of positive cell of GAP -43 and Positive particles area of the treatment group in 15 d and 30 d are significantly increased,compared to the model group and the sham - operated group.Longa evaluation showed significant difference among model group and treatment group in Day15.Longa evaluation of treatment group was better than thoes of model group.Conclusions Exogenous VEGF could enhance angiogenesis and up - regulate the expressions of GAP - 43 after intracerebral hemorrhage.It could reduce Neurotrosis caused by intracerebral hemorrhage and improve neurological function.