CAJ | 학술논문

目的:观察电针对肝硬化大鼠肝组织病理学及血清学指标的影响,初步探讨电针抗大鼠肝硬化的作用及其机制.方法:采用四氯化碳诱导大鼠肝硬化模型,随机分为电针组、秋水仙碱组和模型组,并与正常大鼠作对照,应用形态学、放射免疫分析技术,检测各组大鼠肝组织胶原增生情况及血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PⅢNP)、IV型胶原(C-Ⅳ)的变化.结果:与正常组比较,模型组大鼠肝组织胶原增生,血清HA、LN、PⅢNP含量明显增加;与模型组比较,电针组大鼠肝组织胶原面积及血清HA、LN含量明显减少,血清PⅢNP、C-Ⅳ虽有减少趋势但无统计学意义.结论:电针对大鼠实验性肝硬化具有一定的防治作用,其作用机制可能与电针能够保护肝细胞、抑制细胞外基质的合成和促进胶原产物的代谢有关.
목적:관찰전침대간경화대서간조직병이학급혈청학지표적영향,초보탐토전침항대서간경화적작용급기궤제.방법:채용사록화탄유도대서간경화모형,수궤분위전침조、추수선감조화모형조,병여정상대서작대조,응용형태학、방사면역분석기술,검측각조대서간조직효원증생정황급혈청투명질산(HA)、층점련단백(LN)、Ⅲ형전효원(PⅢNP)、IV형효원(C-Ⅳ)적변화.결과:여정상조비교,모형조대서간조직효원증생,혈청HA、LN、PⅢNP함량명현증가;여모형조비교,전침조대서간조직효원면적급혈청HA、LN함량명현감소,혈청PⅢNP、C-Ⅳ수유감소추세단무통계학의의.결론:전침대대서실험성간경화구유일정적방치작용,기작용궤제가능여전침능구보호간세포、억제세포외기질적합성화촉진효원산물적대사유관.
Objective: To observe the effects of electroacupuncture on the histopathological changes and serum indexes of rats' liver, and to investigate the mechanism of electroacupuncture in treating hepatic fibrosis of rats. Methods: The rat model of hepatic fibrosis was induced with carbon tetrachloride. Then, the rats were divided into electroacupuncture group, medicine group, and model group. The collagen of liver, and serum hyaluronic acid (HA), laminin (LN), procollagen (PⅢNP), and collagen Ⅳ (C-Ⅳ) were detected with morphologic methods and radioimmunoassay. Results: Compared with normal rats, the collagen was hyperplasia in the liver tissue, and the contents of the serum HA, LN, and PⅢ NP were higher in the model group. The collagen area of liver tissue, and the contents of the serum HA, and LN were lower in rats treated with electroacupuncture than model rats. The contents of serum of PⅢ NP, and C-Ⅳ changed little. Conclusion: Electroacupuncture had some effects of prevention and treatment, and the mechanism may relate to the effects of electroacupuncture in protecting liver cells, inhibiting synthesis of extracellular matrix, and promoting the breakup of collagen.