分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2010年
1期
87-90
,共4页
PO_4~(3-)%根际土壤溶液%毛细管电泳
PO_4~(3-)%根際土壤溶液%毛細管電泳
PO_4~(3-)%근제토양용액%모세관전영
Phosphate%Soil solution%Capillary electrophoresis
应用毛细管区带电泳间接紫外吸收法,对测定根际土壤溶液中PO_4~(3-)的检测波长、电泳温度、分离电压和电解液组成等参数进行了选择,发展了根际土壤溶液中PO_4~(3-)浓度的毛细管电泳分析法.选择后的电泳条件为:电解液为32 mmol/L三羟甲基氨基甲烷+4 mmol/L1,2,4-苯三酸+0.3 mmol/L十六烷基三甲基溴化铵(pH=8.5);检测波长为205 nm,分离电压为-20 kV,温度为25℃.本方法有效地屏蔽了土壤溶液中cl~-,SO_4~(2-)和NO_3~-等离子对PO_4~(3-)测定的影响,对根际土壤溶液中PO_4~(3-)的检出限为0.68 mg/L(S/N=3);回收率为87.2%～99.4%,适于测定微量根际土壤溶液样品中PO_4~(3-)的浓度. 细管电泳分析法.选择后的电泳条件为:电解液为32 mmol/L三羟甲基氨基甲烷+4 mmol/L1,2,4-苯i酸+0.3 mmol/L十六烷基三甲基溴化铵(pH=8.5);检测波长为205 nm,分离电压为-20 kV,温度为25℃.本方法有效地屏蔽了土壤溶液中cl~-,SO_4~(2-)和NO_3~-等离子对PO_4~(3-)测 的影响,对根际土壤溶液中PO
應用毛細管區帶電泳間接紫外吸收法,對測定根際土壤溶液中PO_4~(3-)的檢測波長、電泳溫度、分離電壓和電解液組成等參數進行瞭選擇,髮展瞭根際土壤溶液中PO_4~(3-)濃度的毛細管電泳分析法.選擇後的電泳條件為:電解液為32 mmol/L三羥甲基氨基甲烷+4 mmol/L1,2,4-苯三痠+0.3 mmol/L十六烷基三甲基溴化銨(pH=8.5);檢測波長為205 nm,分離電壓為-20 kV,溫度為25℃.本方法有效地屏蔽瞭土壤溶液中cl~-,SO_4~(2-)和NO_3~-等離子對PO_4~(3-)測定的影響,對根際土壤溶液中PO_4~(3-)的檢齣限為0.68 mg/L(S/N=3);迴收率為87.2%～99.4%,適于測定微量根際土壤溶液樣品中PO_4~(3-)的濃度. 細管電泳分析法.選擇後的電泳條件為:電解液為32 mmol/L三羥甲基氨基甲烷+4 mmol/L1,2,4-苯i痠+0.3 mmol/L十六烷基三甲基溴化銨(pH=8.5);檢測波長為205 nm,分離電壓為-20 kV,溫度為25℃.本方法有效地屏蔽瞭土壤溶液中cl~-,SO_4~(2-)和NO_3~-等離子對PO_4~(3-)測 的影響,對根際土壤溶液中PO
응용모세관구대전영간접자외흡수법,대측정근제토양용액중PO_4~(3-)적검측파장、전영온도、분리전압화전해액조성등삼수진행료선택,발전료근제토양용액중PO_4~(3-)농도적모세관전영분석법.선택후적전영조건위:전해액위32 mmol/L삼간갑기안기갑완+4 mmol/L1,2,4-분삼산+0.3 mmol/L십륙완기삼갑기추화안(pH=8.5);검측파장위205 nm,분리전압위-20 kV,온도위25℃.본방법유효지병폐료토양용액중cl~-,SO_4~(2-)화NO_3~-등리자대PO_4~(3-)측정적영향,대근제토양용액중PO_4~(3-)적검출한위0.68 mg/L(S/N=3);회수솔위87.2%～99.4%,괄우측정미량근제토양용액양품중PO_4~(3-)적농도. 세관전영분석법.선택후적전영조건위:전해액위32 mmol/L삼간갑기안기갑완+4 mmol/L1,2,4-분i산+0.3 mmol/L십륙완기삼갑기추화안(pH=8.5);검측파장위205 nm,분리전압위-20 kV,온도위25℃.본방법유효지병폐료토양용액중cl~-,SO_4~(2-)화NO_3~-등리자대PO_4~(3-)측 적영향,대근제토양용액중PO
Analytical method of capillary zone electrophoresis with indirect UV detection was optimized and validated to determine the phosphate in rhizosphere soil solution samples.The effect of detection wavelength, electrolyte composition, separation voltage and temperature were investigated.Analyses were performed in a 56 cm uncoated fused-silica capillary(length to the detector window) with reversed electroosmotic flow, anodic detection in 205 nm, separation voltage-20 kV, and temperature 25 t.The electrolyte contained 32 mmol/L tris-hydroxymethyl-aminomethane, 4 mmol/L 1,2, 4-benzene-tricarboxylic trimellitic acid and 0.3 mmol/L tetradecyl-trimethylammonium bromide, pH 8.5.Under the conditions, the negative effect of the inorganic anion, such as Cl~-, SO_4~(2-) and NO_3~-, can be reduced on phosphate.The detection limit was 0.68 mg/L(S/N=3), and the recovery was 87.2%-99.4%.The method has been applied to the determi nation of the concentration of phosphate in the rhizoshere soil solution.
