CAJ | 학술논문

研究巴东醉鱼草(Buddleja albiflora Hemsl.) 95%乙醇提取物的不同极性溶剂萃取物对3种鳞翅目害虫和朱砂叶螨(Tetranychus cinnabarinus B.)的生物活性.结果表明:巴东醉鱼草95%乙醇提取物的石油醚和乙酸乙酯萃取物对试虫有较高的生物活性,其中乙酸乙酯萃取物在24 h对3龄小菜蛾(Plutella xylostella L.)幼虫的拒食率达到94.22%,48 h时对4龄菜青虫(Pieris rapae L.)幼虫的拒食率达到83.94%,对3龄粘虫(Mythimna separata W.)幼虫的24 h拒食中浓度(AFC_(50))为2.4185 mg·mL~(-1),对朱砂叶螨(T. cinnabarinus) 4 h致死中浓度(LC_(50))为0.2108 mg·mL~(-1);石油醚萃取物72 h对3龄小菜蛾(P. xylostella)幼虫的拒食率为64.11%,48 h对4龄菜青虫(P. rapae)幼虫的拒食率为75.18%,对3龄粘虫(M. separata)幼虫48 h的AFC_(50)值最高(1.1392 mg·mL~(-1)),对朱砂叶螨(T. cinnabarinus) 4 h LC_(50)为 0.3606 mg·mL~(-1);巴东醉鱼草95%乙醇提取物的不同极性溶剂萃取物还抑制试虫的生长发育,导致试虫化蛹和羽化不正常,其中石油醚萃取物和乙酸乙酯萃取物的作用尤为突出.
연구파동취어초(Buddleja albiflora Hemsl.) 95%을순제취물적불동겁성용제췌취물대3충린시목해충화주사협만(Tetranychus cinnabarinus B.)적생물활성.결과표명:파동취어초95%을순제취물적석유미화을산을지췌취물대시충유교고적생물활성,기중을산을지췌취물재24 h대3령소채아(Plutella xylostella L.)유충적거식솔체도94.22%,48 h시대4령채청충(Pieris rapae L.)유충적거식솔체도83.94%,대3령점충(Mythimna separata W.)유충적24 h거식중농도(AFC_(50))위2.4185 mg·mL~(-1),대주사협만(T. cinnabarinus) 4 h치사중농도(LC_(50))위0.2108 mg·mL~(-1);석유미췌취물72 h대3령소채아(P. xylostella)유충적거식솔위64.11%,48 h대4령채청충(P. rapae)유충적거식솔위75.18%,대3령점충(M. separata)유충48 h적AFC_(50)치최고(1.1392 mg·mL~(-1)),대주사협만(T. cinnabarinus) 4 h LC_(50)위 0.3606 mg·mL~(-1);파동취어초95%을순제취물적불동겁성용제췌취물환억제시충적생장발육,도치시충화용화우화불정상,기중석유미췌취물화을산을지췌취물적작용우위돌출.
The insecticidal activities of the extraction fractions, which were obtained by soaking the powder of Buddleja albiflora H. with 95% ethanol and then treated with petroleum ether, ethyl acetate and butanol, against three kinds of pests of Lepidoptera and Tetranychus cinnabarinus B. were tested. The results showed that the petroleum and ethyl acetate extracts possessed considerable activities to all testing pests. The antifeedant rates of ethyl acetate extracts against 3rd instar larvae of Plutella xylostella L. during 24 h and 4th instar larvae of Pieris rapae L. during 48 h were 94.22% and 83.94%, respectively. The median antifeedant concentration (AFC_(50)) was 2.4185 mg·mL~(-1) against Mythimna separata W. during 24 h and the median-lethal concentration (LC_(50)) was 0.2108 mg·mL~(-1) against T. cinnabarinus. The antifeedant rates of petroleum ether extracts against 3rd instar larvae of P. xylostella during 72 h and 4th instar larvae of P. rapae during 48 h were 64.11% and 75.18%, respectively. The AFC_(50) was 1.1392 mg·mL~(-1) against M. separata during 48 h and the LC_(50) was 0.3606 mg·mL~(-1) against T. cinnabarinus during 4 h. The bioassay results also showed the extraction fractions of B. albiflora, especially the petroleum ether extracts and ethyl acetate one, cound inhibit the growth and development of insects, leading to abnormal pupation and eclosion finally.